孕妇肠道菌群对新生儿出生体质量的影响研究  

Effect of Maternal Intestinal Floras on Neonatal Birth Weight

作　　者：肖雪 王宇琦 赖晓岚 钟绍涛 赵翠柳 刘丹 李琳 谭荣韶 杨红玲 XIAO Xue;WANG Yuqi;LAI Xiaolan;ZHONG Shaotao;ZHAO Cuiliu;LIU Dan;LI Lin;TAN Rongshao;YANG Hongling(Guangzhou Red Cross Hospital,Guangzhou 510220,China;Guangzhou Women and Children's Medical Center,Guangzhou 510623,China)

机构地区：[1]广州红十字会医院,广东省广州市510220 [2]广州市妇女儿童医疗中心,广东省广州市510623

出　　处：《中国全科医学》2022年第19期2363-2370,共8页Chinese General Practice

基　　金：广东省科技计划项目(2017B090904027);中国营养学会青年人才项目(2020-51号);广州市卫生和计划生育科技项目(2018A010015,20211A010017)。

摘　　要：背景新生儿出生体质量与个体健康息息相关,低出生体质量是早期新生儿死亡的高危因素,而巨大儿的发生不仅可增加母婴产时并发症发生风险,还可增加个体成年后罹患各种慢性病的风险。因此,寻找新生儿出生体质量的影响因素十分重要。目的探讨孕妇肠道菌群对新生儿出生体质量的影响。方法以2017年1—9月在广州市妇女儿童医疗中心出生的516例新生儿及其孕母为研究对象,根据新生儿出生体质量将其分为低出生体质量儿组(LW组,n=24)、正常体质量儿组(NW组,n=479)、巨大儿组(OW组,n=13)。采集孕母的肠道菌群参数及临床实验室检测指标,采用QIIME软件进行孕期肠道菌群组成分析和多样性分析;采用LEfSe分析,分别对三组孕妇肠道菌群属水平上的相对丰度进行两两比较,识别组间具有明显差异的菌群;通过线性模型MaAsLin进行多元分析,以捕获各实验室检测指标与微生物属之间的相关性;通过Boruta随机森林分类器模型分别基于实验室检测指标和肠道菌群分类操作单元(OTUs)构建新生儿出生体质量分类预测模型,探究孕妇肠道菌群对新生儿体质量的影响。结果三组孕母的肠道菌群组成分析发现,门水平中厚壁菌门(Firmicutes)物种丰富度最高,属水平里普拉梭菌(Faecalibacterium)明显富集,三组间门水平的香农指数和辛普森指数比较,差异无统计学意义(P>0.05)。三组间的LEfSe分析发现:与LW组比较,NW组链球菌(Streptococcus)和罗氏菌(Roseburia)明显富集(P<0.05),而芽孢杆菌(Bacillaceae)、萝卜属菌(Raphanus)、甲烷球形菌(Methanosphaera)、巴氏杆菌(Barnesiellaceae)、普雷沃氏菌(Paraprevotella)丰度明显降低(P<0.05);与NW组比较,OW组属巨单胞菌(Megamonas)、属粪球菌(Coprococcus)、韦荣氏菌(Veillonellaceae)、cc-115、梭菌(Closrtidiaceae)、另枝杆菌(Alistipes)明显富集(P<0.05),而布劳特氏菌(Blautia)和伊格尔兹氏菌(Eggerthella)丰�Background Birth weight is closely related to individual health.Low birth weight is a high-risk factor for early neonatal death.Macrosomia is associated with higher risk of maternal and infant complications and various chronic diseases in adulthood.Therefore,it is very important to identify the influencing factors of neonatal birth weight.Objective To investigate the relationship between maternal intestinal flora and neonatal birth weight.Methods Participants were 516 singleton mothers and their babies〔24 with low birth weight(LW group),479 with normal birth weight(NW group)and 13 with macrosomia(OW group)〕delivered at term in Guangzhou Women and Children's Medical Center from January to September 2017.Maternal intestinal flora and clinical laboratory test parameters were collected,and the composition and diversity of intestinal flora were analyzed using QIIME.LEfSe analysis was used to compare the relative abundance of intestinal flora at the genus level of the mothers of three groups of babies to identify the flora with significant intergroup differences.MaAslin was used to assess associations of maternal laboratory test parameters and microbial genera.The Boruta was used to build models for predicting three types of neonatal birth weight using maternal laboratory test parameters and intestinal flora OTUs,to assess the association of maternal intestinal floras and neonatal birth weight.Results The analysis of maternal intestinal floras showed that the abundance of Firmicutes was the highest at the phylum level,and Faecalibacterium was significantly enriched at the genus level.There were no significant differences in Simpson's Diversity Index and Shannon Diversity Index at the phylum level across the maternal intestinal floras of three groups of babies(P>0.05).LEfSe analysis found that compared with intestinal flora of mothers of LW group,the intestinal flora of mothers of NW group showed significantly enriched Streptococcus and Roseburia(P<0.05),and significantly reduced abundance of Bacillaceae,Raphanus,Me

关 键 词：婴儿健康 出生体重 胃肠道微生物组 LEfSe分析 随机森林 

分 类 号：R722[医药卫生—儿科] R174[医药卫生—临床医学]