海洋真菌Penicillium sp.WP-13次级代谢产物研究  

Secondary metabolites of marine-derived fungus Penicillium sp.WP-13

作　　者：王慧芳 梅文莉[2] 盖翠娟 戴好富[2] 王佩 谭志琼[1] WANG Huifang;MEI Wenli;GAI Cuijuan;DAI Haofu;WANG Pei;TAN Zhiqiong(School of Life Sciences,Hainan University,Haikou 570228,Hainan,China;Key Laboratory of Natural Products Research and Development of Li Folk Medicine of Hainan Province,Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,Hainan,China)

机构地区：[1]海南大学生命科学学院,海南海口570228 [2]中国热带农业科学院热带生物技术研究所海南省黎药资源天然产物研究与利用重点实验室,海南海口571101

出　　处：《菌物学报》2022年第5期782-791,共10页Mycosystema

基　　金：海南省自然科学基金(320QN335);农业农村部财政专项项目(NFZX2021)。

摘　　要：本研究分析了海洋真菌Penicillium sp.WP-13的活性次级代谢产物。采用多种柱色谱技术对其发酵液的乙酸乙酯提取物进行分离纯化;根据波谱数据和理化常数分析,并结合文献比对鉴定单体化合物的结构;采用MTT法测定化合物对肿瘤细胞的细胞毒活性。从Penicillium sp.WP-13发酵液的乙酸乙酯提取物中分离鉴定了5个单体化合物,其中2个内酯类化合物为1-hydroxy-3,10-dimethoxy-8-methyl-6,11-dioxo-6,11-dihydrodibenzo[b,e]oxepine-9-carboxylicacid(1)和1,8-dihydroxy-10-methoxy-3-methyl-dibenzo[b,e]oxepine-6,11-dione(2);3个蒽醌类化合物为endocrocinmethylester(3)、2-chloro-1,3,8-trihydroxy-6-methyl-9,10-anthracenedione(4)和emodin(5)。活性测试结果显示,化合物3和5均对人慢性髓原白血病细胞株K562、人肝癌细胞株BEL-7402、人胃癌细胞株SGC-7901和人宫颈癌细胞株HeLa具有一定的细胞毒活性。化合物1为新化合物,化合物3的核磁数据为首次报道,化合物1–4均首次分离自青霉属真菌。The active secondary metabolites of the marine-derived fungus Penicillium sp.WP-13were investigated.The secondary metabolites were isolated and purified by various column chromatography techniques,and the structures of the compounds were identified by analysis of spectral data and physicochemical constants combined with literature comparison.The cytotoxic activity of the compounds was evaluated by MTT assay.Five compounds including two lactones and three anthraquinones were isolated from the ethyl acetate extract of the fermentation broth of Penicillium sp.WP-13.Their structures were determined as 1-hydroxy-3,10-dimethoxy-8-methyl-6,11-dioxo-6,11-dihydrodibenzo[b,e]oxepine-9-carboxylic acid(1),1,8-dihydroxy-10-methoxy-3-methyl-dibenzo[b,e]oxepine-6,11-dione(2),endocrocin methyl ester(3),2-chloro-1,3,8-trihydroxy-6-methyl-9,10-anthracenedione(4),and emodin(5).The results of cytotoxic test showed that compounds 3 and 5 displayed cytotoxic activity against human chronic myeloid leukemia cell line K562,human liver cancer cell line BEL-7402,human gastric cancer cell line SGC-7901 and human cervical cancer cell line HeLa.Compound 1 is a new lactone,and the NMR data of compound 3 were reported for the first time.Compounds 1–4 were isolated from Penicillium for the first time.

关 键 词：海洋真菌 青霉属 次级代谢产物 细胞毒活性 

分 类 号：Q936[生物学—微生物学]