FcεR1α基因多态性与过敏性鼻炎的关系分析  

Analysis of relationship between FcεR1α gene polymorphisms and allergic rhinitis

作　　者：焦红叶 吴明海 施涛 许莉 陈伟 程友 JIAO Hongye;WU Minghai;SHI Tao;XU Li;CHEN Wei;CHENG You(Department of Otolaryngology Head and Neck Surgery,General Hospital of Eastern Theater Command,Nanjing,Jiangsu 210002,China)

机构地区：[1]东部战区总医院耳鼻咽喉头颈外科,江苏南京210002

出　　处：《检验医学与临床》2022年第11期1454-1457,共4页Laboratory Medicine and Clinic

基　　金：江苏省自然科学基金项目(BK20161388)。

摘　　要：目的分析免疫球蛋白(Ig)E高亲和力受体α段(FcεR1α)基因rs2427827位点的单核苷酸多态性(SNP)分布情况,并探讨其与过敏性鼻炎(AR)的关系。方法采取病例对照研究方法,选择2019年1月至2020年7月于该院耳鼻咽喉头颈外科就诊并确诊为AR的151例患者为AR组,选取同期健康体检志愿者108例为对照组。采集所有受试者外周抗凝血3 mL并提取血液DNA,采用Mass ARRAY iPLEX GOLD分型技术检测两组FcεR1α基因rs2427827位点多态性;采用Logistic回归分析影响AR易感性的相关因素。结果AR组FcεR1α基因rs2427827位点CC基因型频率为66.2%,尽管高于对照组的65.7%,但是差异无统计学意义(P>0.05)。Logistic回归分析结果显示,突变杂合基因型TC(P=0.226,OR=2.15,95%CI:0.63~7.85)与突变纯合基因型CC(P=0.262,OR=1.97,95%CI:0.61~6.90)均与AR的发病无关。突变等位基因型C与野生等位基因型T进行比较,发现没有增加AR的患病风险,差异无统计学意义(P=0.603,OR=1.12,95%CI:0.72~1.75)。结论FcεR1α基因多态性与AR无明显相关性,但由于样本条件的限制,有必要扩大样本量和样本选择范围,以便进一步研究。Objective To analyze theαsegment of IgE high-affinity receptor(FcεR1α)gene single nucleotide polymorphisms(SNP)at the rs2427827 site,and to explore its relationship with allergic rhinitis(AR).Methods By using the case-control group method,151 patients with AR diagnosed in the otolaryngology head and neck surgery department of this hospital from January 2019 to July 2020 were selected as the AR group,and 108 healthy volunteers undergoing physical examination at the same period were selected as the control group.Three mL of anticoagulant peripheral blood was collected in all subjects and blood DNA was extracted,the FcεR1α gene polymorphisms at rs2427827 site in the two groups were detected by Mass ARRAY iPLEX GOLD typing technique;the Logistic regression was used to analyze the relevant factors affecting the AR susceptibility.Results The CC genotype frequency of FcεR1α gene rs2427827 site was 66.2%,although which was higher than that in the control group by 65.7%,but the difference was not statistically significant(P>0.05);the Logistic regression analysis results revealed that the mutant heterozygous genotype TC(P=0.226,OR=2.15,95%CI:0.63-7.85)and the mutant homozygous genotype CC(P=0.262,OR=1.97,95%CI:0.61-6.90)had no correlation with the onset of AR.The comparison between mutant alleltype C and wild alleltype T found that the risk suffering from AR was not increased,and the difference was not statistically significant(P=0.603,OR=1.12,95%CI:0.72-1.75).Conclusion The FcεR1α gene SNP has no significant correlation with AR,but due to the limitations of sample conditions,it is necessary to expand the sample size and sample selection range for further investigation.

关 键 词：单核苷酸多态性 免疫球蛋白E高亲和力受体α段 过敏性鼻炎 免疫球蛋白E 

分 类 号：R765.21[医药卫生—耳鼻咽喉科]