流动注射化学发光法测定阿卡波糖  

Flow injection chemiluminescence method for determination of acarbose

作　　者：赵冕 王贝贝[1] 王冰 李献锐 ZHAO Mian;WANG Beibei;WANG Bing;LI Xianrui(Experimental Center for Teaching, Hebei Medical University, Shijiazhuang 050017, China;College of Pharmacy,Hebei Medical University, Shijiazhuang 050017,China)

机构地区：[1]河北医科大学教学实验中心,河北石家庄050017 [2]河北医科大学药学院,河北石家庄050017

出　　处：《河北大学学报（自然科学版）》2022年第3期251-255,共5页Journal of Hebei University(Natural Science Edition)

基　　金：河北省自然科学基金资助项目(B2020206009);河北省高等学校科学技术研究项目(QN2021101)。

摘　　要：建立一种流动注射化学发光法测定阿卡波糖的新方法.在碱性条件下,阿卡波糖对H_(2)O_(2)-鲁米诺发光体系的发光强度具有显著的增敏作用,在最佳实验条件下,发光强度的增加值(ΔI)与阿卡波糖的质量浓度在3.0×10^(-4)~2.0×10^(-2)mg/mL内成线性关系,相关系数为0.9987.方法的检出限为2.3×10^(-4)mg/mL,对0.01 mg/mL的阿卡波糖连续测定11次,其相对标准偏差为2.7%,并应用于阿卡波糖片剂含量的测定,平均回收率为101.6%.本方法操作简单快速,重现性好,可用于阿卡波糖的含量测定.A simple and rapid flow-injection chemiluminescence(FI-CL)method was developed for the determination of acarbose in tablets based on the fact that acarbose can remarkably enhance the chemiluminescence of Luminol-H_(2)O_(2)in alkaline medium.The determination conditions were optimized.Under optimum conditions,the chemiluminescence relative intensity had a good linear relationship with acarbose concentration in the range of 3×10^(-4)~2×10^(-2)mg/mL with a correlation coefficient of 0.9987.The detection limit was 2.3×10^(-4)mg/mL and RSD(n=11)is 2.7%for 0.01 mg/mL acarbose.The proposed method has been successfully applied for the determination of acarbose in tablets sample and the average recovery was 101.6%.

关 键 词：阿卡波糖 流动注射 化学发光 增敏 

分 类 号：O657.39[理学—分析化学]