转录因子LMO3对子宫内膜癌患者预后的影响  

Significance of transcription factor LMO3 in evaluating prognosis of patients with endometrial carcinoma

作　　者：刘佳丽[1] 宣贝贝 全权 龚赛楠 牟晓玲[1] LIU Jiali;XUAN Beibei;QUAN Quan;GONG Sainan;MU Xiaoling(Department of Obstetrics,the First Affiliated Hospital of Chongqing Medical University,Chongqing,400016,China)

机构地区：[1]重庆医科大学附属第一医院妇科,重庆400016

出　　处：《陆军军医大学学报》2022年第10期1054-1060,1073,共8页Journal of Army Medical University

摘　　要：目的探讨转录因子LMO3表达水平对子宫内膜癌患者预后的影响。方法应用TCGA、GeneMANIA、STRING数据库分析子宫内膜癌组织中LMO3表达与患者预后的关系,以及LMO3的基因互作关系、LMO3与TP53突变的关系。收集51例2011年3月至2014年12月在重庆医科大学附属第一医院接受手术且组织病理学诊断为子宫内膜癌患者的子宫内膜组织石蜡切片及临床信息,并进行免疫组织化学染色,分析LMO3在子宫内膜癌组织中的表达及其与子宫内膜癌患者临床病理特征的关系。利用SPSS绘制生存曲线及COX多因素进行回归分析。在子宫内膜癌细胞中过表达LMO3后验证其对肿瘤细胞迁移的影响。结果TCGA数据库分析结果显示,在子宫内膜癌患者中LMO3高表达组的总生存期和无病生存期均显著低于LMO3低表达组(P<0.05),LMO3在TP53突变型患者中的表达水平显著高于TP53野生型患者,通过GeneMANIA及STRING数据库分析发现与LMO3相互作用的基因主要有NHLH2、LDB2等。免疫组织化学染色结果显示LMO3高表达组患者的无病生存期低于LMO3低表达组(P=0.005),多因素COX分析提示LMO3可作为子宫内膜癌的预后因素。过表达LMO3后,子宫内膜癌细胞的迁移能力增强。结论LMO3高表达的子宫内膜癌患者预后差,其可能与LMO3过表达促进子宫内膜癌细胞的迁移有关。Objective To investigate the significance of transcription factor LIM domain only 3(LMO3)expression level in the prognosis of patients with endometrial carcinoma(EC).Methods The Cancer Genome Atlas(TCGA),GeneMANIA and STRING databases were used to analyze the relationship between LMO3 expression in EC tissues and the prognosis of patients,as well as the genes interacting with LMO3,and the correlation of LMO3 with TP53 mutation.The paraffin sections and clinical information of 51 patients with EC who underwent surgery in our hospital from March 2011 to December 2014 were collected.Immunohistochemical staining was subsequently performed to determine the expression of LMO3 in EC tissues and its association with the clinicopathological features of patients.SPSS was used to plot the survival curve,and COX multivariate regression analysis was also adopted.In addition,LMO3 overexpression was conducted in EC cells to verify its effect on tumor cell migration.Results According to the analysis of TCGA database,the overall survival and disease-free survival of patients with high LMO3 expression were significantly shorter than those of low LMO3 expression(P<0.05),and the expression level of LMO3 in patients with TP53 mutation was remarkably higher than that in TP53 wild-type patients.GeneMANIA and STRING analyses showed that the main genes interacting with LMO3 included NHLH2 and LDB2.Moreover,immunohistochemical staining also indicated that patients with higher LMO3 expression level had shorter disease-free survival(P=0.005),and multivariate COX analysis suggested LMO3 as a prognostic factor for EC.Finally,overexpression of LMO3 enhanced the migration of EC cells.Conclusion EC patients with high LMO3 level have a poor prognosis,which may be related to the migration of EC cells promoted by LMO3 overexpression.

关 键 词：转录因子 LMO3 子宫内膜癌 预后 生物信息学 

分 类 号：R341[医药卫生—基础医学] R730.7