人早孕蜕膜基质细胞表达的SGK1对育龄女性外周血Treg/Th17平衡的影响  

Effects of SGK1 on Treg/Th17 balance in the peripheral blood of women of reproductive age

作　　者：刘晴晴 刘福民 刘小云 冯霞 林华 王风兰 LIU Qingqing;LIU Fumin;LIU Xiaoyun;FENG Xia;LIN Hua;WANG Fenglan(Graduate School,Xuzhou Medical University,Xuzhou,Jiangsu 221004,China;Department of Obstetrics and Gynecology,the Affiliated Hospital of Xuzhou Medical University,Xuzhou,Jiangsu 221002;Central Laboratory,the Affiliated Hospital of Xuzhou Medical University)

机构地区：[1]徐州医科大学研究生院,江苏徐州221004 [2]徐州医科大学附属医院妇产科,江苏徐州221002 [3]徐州医科大学附属医院中心实验室

出　　处：《徐州医科大学学报》2022年第5期349-352,共4页Journal of Xuzhou Medical University

基　　金：徐州市科技局社会发展项目(KC16SL108)。

摘　　要：目的研究蜕膜基质细胞(DSCs)中表达的SGK1对育龄女性外周血淋巴细胞Treg/Th17平衡的影响。方法分离培养正常人早孕DSCs,免疫细胞化学鉴定DSCs,体外分离培养育龄期女性外周血淋巴细胞(PBLC),并分别与各组DSCs共培养。实验分为5组:对照组、共培养组、SGK1激活剂组、SGK1抑制剂组、共刺激组。Western blot检测各组SGK1蛋白表达水平;逆转录聚合酶链(RT-PCR)法检测SGK1 mRNA表达水平;流式细胞术检测各组Treg与Th17细胞亚群比例。结果Western blot和RT-PCR结果显示,SGK1激活剂组SGK1蛋白以及mRNA表达明显高于其余各组(P<0.05);与共培养组相比,SGK1抑制剂组和共刺激组SGK1蛋白和mRNA表达明显降低(P<0.05),且抑制剂组降低更明显(P<0.05)。流式细胞术检测显示,SGK1激活剂组Treg细胞亚群/Th17细胞亚群比例明显高于其余4组(P<0.05);与对照组相比,共培养组Treg细胞比例升高且Treg/Th17比例也明显增加;与共培养组相比,SGK1抑制剂组和共刺激组Treg细胞亚群比例明显降低(P<0.05),且SGK1抑制剂组降低更明显(P<0.05)。结论人早孕DSCs表达的SGK1能够影响外周血淋巴细胞中Treg亚群和Th17亚群的比例。Objective To investigate the effect of SGK1 in decidual stromal cells(DSCs)on Treg/Th17 balance in the peripheral blood lymphocytes of women of reproductive age.Methods DSCs isolated from normal early pregnant women were cultured and identified by immunocytochemistry.The peripheral blood lymphocytes(PBLC)of women of reproductive age were isolated,and co-cultured with DSCs.There were five groups in the current study:a control group,a co-culture group,a SGK1 agonist group,a SGK1 inhibitor group,and a co-stimulation group.The levels of SGK1 were detected by Western blot.The levels of SGK1 mRNA were detected by RT-PCR.The proportion of Treg and Th17 cells was measured by flow cytometry.Results Western blot and RT-PCR results indicated that the levels of SGK1 protein and mRNA in the SGK agonist group were significantly higher than those in other groups(P<0.05).Compared with the co-culture group,the levels of SGK1 protein and mRNA in the SGK1 inhibitor group and the co-stimulation group remarkably decreased(P<0.05),where decreases in the SGK1 inhibitor group were more obvious(P<0.05).According to flow cytometry,the ratio of Treg cells/Th17 cells in the SGK1 agonist group was significantly higher than those in the other four groups(P<0.05).Compared with the control group,the proportion of Treg and Treg/Th17 ratio in in the co-culture group dramatically increased;compared with the co-culture group,the proportion of Treg subsets in the SGK1 inhibitor group and the co-stimulus group was obviously reduced(P<0.05),where decreases in the SGK1 inhibitor group were more obvious(P<0.05).Conclusions SGK1 expressed by DSCs in early pregnancy can affect the proportion of Treg subgroup and Th17 subgroup in peripheral blood lymphocytes。

关 键 词：SGK1 淋巴细胞 调节性T细胞 辅助性T细胞 免疫耐受 

分 类 号：R711.5[医药卫生—妇产科学]