高效液相色谱法同时测定前胡药材中白花前胡甲素和白花前胡乙素含量  

Simultaneous Determination of Praeruptorin A and Praeruptorin B in Peucedani Radix by HPLC

作　　者：邱明鸣 朱育凤[1] 倪昌荣[1] QIU Mingming;ZHU Yufeng;NI Changrong(Jiangsu Province Hospital of Chinese Medicine,Nanjing,Jiangsu,China 210029)

机构地区：[1]江苏省中医院,江苏南京210029

出　　处：《中国药业》2022年第11期70-73,共4页China Pharmaceuticals

基　　金：江苏省中医院2021年度院级课题药学部专项项目[Y21033]。

摘　　要：目的建立同时测定前胡药材中指标性成分白花前胡甲素和白花前胡乙素含量的高效液相色谱法。方法共收集安徽、浙江、四川、湖南等地产的31批前胡药材样品,色谱柱为Agilent Eclipse XDB-C_(18)柱(250 mm×4.6 mm,5μm),流动相为水-甲醇(25∶75,V/V),流速为1 mL/min,柱温为25℃,检测波长为321 nm,进样量为10μL。结果白花前胡甲素和白花前胡乙素的质量浓度分别在3.8802~248.3333μg/mL(r=0.9998,n=5)和1.8880~120.8333μg/mL(r=0.9999,n=5)范围内与峰面积线性关系良好;精密度、稳定性、重复性试验的RSD均小于2.0%;平均加样回收率分别为98.93%和99.66%,RSD分别为1.64%和1.79%(n=6)。白花前胡甲素和白花前胡乙素的含量分别为0.62%~1.63%和0.06%~0.59%;按2020年版《中国药典(一部)》规定,白花前胡甲素含量合格的样品有24批(77.42%),白花前胡乙素含量合格的样品有10批(32.26%),2种成分含量均合格的样品仅9批(29.03%)。结论该方法操作简便、结果准确、重复性好,可用于同时测定前胡药材中白花前胡甲素和白花前胡乙素的含量。市售前胡药材质量参差不齐,合格率低,需要一个切合实际的质量控制方法来评价、控制其质量。Objective To establish a high-performance liquid chromatography(HPLC)method for the simultaneous determination of the index ingredients including praeruptorin A and praeruptorin B in Peucedani Radix.Methods Thirty-one batches of Peucedani Radix samples from Anhui,Zhejiang,Sichuan and Hunan were collected.The chromatographic column was Agilent Eclipse XDB-C_(18) column(250 mm×4.6 mm,5μm),the mobile phase was water-methanol(25∶75,V/V),the flow rate was 1 mL/min,the column temperature was 25℃,the detection wavelength was 321 nm and the injection volume was 10μL.Results The linear ranges of praeruptorin A and praeruptorin B were 3.8802-248.3333μg/mL(r=0.9998,n=5)and 1.8880-120.8333μg/mL(r=0.9999,n=5),respectively.The RSDs of precision,stability and repeatability tests were less than 2.0%.The average recoveries of praeruptorin A and praeruptorin B were 98.93%and 99.66%with RSDs of 1.64%and 1.79%(n=6),respectively.The contents of praeruptorin A and praeruptorin B were 0.62%-1.63%and 0.06%-0.59%,respectively.There were 24 batches(77.42%)of samples with qualified content of praeruptorin A,10 batches(32.26%)of samples with qualified content of praeruptorin B and only nine batches(29.03%)of samples with qualified content of the above two ingredients according to the requirements of the Chinese Pharmacopoeia(2020 Edition,VolumeⅠ).Conclusion The method is simple,accurate and reproducible,which can be used for the simultaneous determination of praeruptorin A and praeruptorin B in Peucedani Radix.The commercially available Peucedani Radix has uneven quality and low qualified rate,which needs a practical quality control method to evaluate and control its quality.

关 键 词：前胡药材 高效液相色谱法 白花前胡甲素 白花前胡乙素 含量测定 

分 类 号：R932[医药卫生—生药学] R284.1[医药卫生—药学]