铜滤膜基环丙沙星分子印迹复合膜的制备、吸附性能及表面增强拉曼散射活性研究  

Preparation and Adsorption Properties of Ciprofloxacin Molecularly Imprinted Composite Membrane with SERS Activity Based on Copper Filter Membrane

作　　者：张杰[1] 唐丽 卜明 马玉坤 王海君[1] 王静[1] ZHANG Jie;TANG Li;BU Ming;MA Yu-kun;WANG Hai-jun;WANG Jing(College of Pharmacy,Qiqihar Medical University,Qiqihar 161006,China;Research Institute of Medicine&Pharmacy,Qiqihar Medical University,Qiqihar 161006,China)

机构地区：[1]齐齐哈尔医学院药学院,黑龙江齐齐哈尔161006 [2]齐齐哈尔医学院医药科学研究院,黑龙江齐齐哈尔161006

出　　处：《化学试剂》2022年第6期850-857,共8页Chemical Reagents

基　　金：黑龙江省省属本科高校基本科研业务费科技项目(2019-KYYWF-1242)。

摘　　要：为实现环丙沙星的快速识别,制备了可以选择性吸附环丙沙星的具有表面增强拉曼活性的铜滤膜基分子印迹复合膜。以环丙沙星为模板分子,铜滤膜为基膜,甲基丙烯酸为功能单体,乙二醇二甲基丙酸酯为交联剂,偶氮二异丁腈为引发剂,甲醇、二氯甲烷的混合溶剂为反应溶剂和致孔剂,通过自由基聚合制备了分子印迹复合膜。红外光谱表征膜的结构,显微镜表征膜的形貌。对膜的吸附性能和表面增强拉曼散射活性进行了系统研究。复合膜为一层白色、连续、均匀的薄膜,表面光滑无裂纹。120 min左右膜对环丙沙星的吸附达到平衡,最大吸附量为8.11μg/cm^(2),至少可以重复使用6次,印迹因子为4.0。膜具有表面增强拉曼散射活性,对环丙沙星检测的最低浓度为1.00×10^(-8)mol/L。In order to realize the rapid recognition of ciprofloxacin(CIP),a molecularly imprinted composite membrane(RC-MIM)with Surface-Enhanced Raman Scattering(SERS)activity based on copper filter membrane was prepared,which could selectively absorb CIP.Using CIP as the template molecule,copper filter membrane as the base membrane,methacrylic acid(MAA)as the functional monomer,and ethylene glycol dimethylpropionate(EGDMA)as the crosslinking agent,azodiisobutyronitrile(AIBN)as the initiator,mixed solvent(methanol and dichloromethane)respectively as the reaction solvent and porogen,RC-MIM was prepared via free radical polymerization.The structure of the RC-MIM was characterized by infrared spectroscopy and the morphology by optical microscope.The adsorption performance and SERS activity of the membrane were systematically studied,revealing that RC-MIM was a white,continuous,and uniform membrane with a smooth surface and free of crack.The adsorption of ciprofloxacin on the membrane reached equilibrium after about 120 min,and the maximum adsorption capacity was 8.11μg/cm^(2).The membrane could be reused at least 6 times,and the imprinting factor was 4.0.Due to the SERS activity of RC-MIM,the minimum detection concentration of ciprofloxacin was 1.00×10^(-8)mol/L.

关 键 词：环丙沙星 分子印迹 复合膜 铜滤膜 表面增强拉曼散射 

分 类 号：O657.1[理学—分析化学]