HPLC测定人血清中三种抗HIV药  

Simultaneous Determination of Three Anti HIV Drugs in Serum by HPLC

作　　者：龚爱琴[1] GONG Ai-qin(Chemical Engineering Institute,Yangzhou Polytechnic Institute,Yangzhou 225127,China)

机构地区：[1]扬州工业职业技术学院化学工程学院,江苏扬州225127

出　　处：《化学试剂》2022年第6期904-907,共4页Chemical Reagents

基　　金：江苏高校“青蓝工程”项目(苏教师[2018]12号);江苏省高等学校优秀科技创新团队项目(苏教科[2017]6号);江苏省大学生实践创新课题项目(202013754024Y);扬州工业职业技术学院高层次人才科研启动经费项目。

摘　　要：建立一种用高效液相色谱法同时测定人血清中3种抗HIV药(拉米夫定、富马酸替诺福韦二吡呋酯和ACC007)的新方法。测定时采用Dionex C_(18)(5μm,4.60 mm×250 mm)色谱柱;流动相为甲醇-水,采用梯度洗脱方式,流速为0.6 mL/min;检测波长为260 nm。结果发现在优化色谱条件下,拉米夫定、富马酸替诺福韦二吡呋酯和ACC007的线性范围分别为2.07~103.5、5.15~206.0、2.06~51.45μg/mL,检出限分别为1.05、2.60、1.03μg/mL,加样回收率分别为98.54%~103.8%、98.29%~102.5%、101.7%~108.4%。本方法简单快速,可用于人血清中3种药物含量的测定。In this work,a new method for the simultaneous determination of lamivudine,tenofovir disoproxil fumarate,and ACC007 in human serum was developed with high-performance liquid chromatography(HPLC).A Dionex C_(18)chromatographic column(5μm,4.60 mm×250 mm)was used with the methanol-water mobile phase(gradient elution)and a flow rate of the mobile phase of 0.6 mL/min.The detection wavelength was 260 nm.Under the optimized chromatographic conditions:the linear ranges of lamivudine,tenofovir disoproxil fumarate and ACC007 were 2.07~103.5,5.15~206.0,2.06~51.45μg/mL,respectively;the detection limits for lamivudine,tenofovir disoproxil fumarate and ACC007 were 1.05,2.60,and 1.03μg/mL,respectively;and accordingly,the recoveries in the spiked samples were 98.54%~103.8%,98.29%~102.5%,and 101.7%~108.4%,respectively.The proposed method is simple and fast and can be used for the determination of these three drugs in human serum.

关 键 词：抗HIV药 拉米夫定 富马酸替诺福韦二吡呋酯 ACC007 HPLC 

分 类 号：O657.7[理学—分析化学]