南海礁栖海藻共附生真菌Pestalotiopsis neglecta SCSIO41403次级代谢产物研究  

Study on the secondary metabolites of reef habitat algae-derived fungus Pestalotiopsis neglecta SCSIO41403 from the South China Sea

作　　者：叶禹秀 罗小卫 杨斌[2,3] 林秀萍 刘永宏[2] 周雪峰[2,3] YE Yuxiu;LUO Xiaowei;YANG Bin;LIN Xiuping;LIU Yonghong;ZHOU Xuefeng(Institute of Marine Drugs,Guangxi University of Chinese Medicine,Nanning 530200,China;CAS Key Laboratory of Tropical Marine Bio-resources and Ecology,Guangdong Key Laboratory of Marine Materia Medica,South China Sea Institute of Oceanology,Chinese Academy of Sciences,Guangzhou 510301,China;Hainan Provincial Key Laboratory of Tropical Marine Biology Technology,Sanya Institute of Oceanology,Chinese Academy of Sciences,Sanya 572024,China)

机构地区：[1]广西中医药大学海洋药物研究院,广西南宁530200 [2]中国科学院热带海洋生物资源与生态重点实验室,广东省海洋药物重点实验室,中国科学院南海海洋研究所,广东广州510301 [3]海南省热带海洋生物技术重点实验室,三亚中科海洋研究院,海南三亚572024

出　　处：《热带海洋学报》2022年第3期186-190,共5页Journal of Tropical Oceanography

基　　金：海南省重大科技计划项目(ZDKJ202018);国家自然科学基金项目(U20A20101、81973235)。

摘　　要：研究南海礁栖腔节藻Coelarthrum sp.共附生真菌Pestalotiopsis neglecta SCSIO41403的次级代谢产物。采用中压正相柱层析色谱法和半制备高效液相色谱法,对真菌发酵产物进行分离纯化,制备获得6个次级代谢产物单体化合物,使用核磁共振、质谱等波谱学方法,结合其理化性质以及文献数据对比,分别鉴定为:gamahorin(1)、microsporaline C(2)、7-hydroxy-5-methoxy-4,6-dimethylphthalidc(3)、demethyl nectriapyrone A(4)、4-hydroxybenzaldehyde(5)和4-hydroxyphenethyl acetate(6)。这6个化合物均为首次从该菌株发现,其中化合物1、2、5和6是首次从海洋动植物共附生真菌中发现。抗菌活性筛选显示其中化合物3对白色念珠菌有明显抑制活性。We research on the secondary metabolites from the fungus Pestalotiopsis neglecta SCSIO41403,derived from the coral reefs habitat algae Coelarthrum sp.in the South China Sea.Six pure secondary metabolites were isolated and purified by medium pressure normal phase column chromatography and semi-preparative high performance liquid chromatography(HPLC)in the fungal culture broth.The compounds were identified and elucidated as gamahorin(1),microsporaline C(2),7-hydroxy-5-methoxy-4,6-dimethylphthalidc(3),demethyl nectriapyrone A(4),4-hydroxybenzaldehyde(5),4-hydroxyphenethyl acetate(6)by nuclear magnetic resonance spectrum(NMR),mass spectrum(MS),and literature analysis.All compounds were obtained from this strain for the first time,and compounds 1,2,5,and 6 were discovered from marine-derived fungus for the first time.In the antibacterial tests,compound 3 was revealed to have significant inhibitory activity against Candida albicans.

关 键 词：海藻共附生真菌 Pestalotiopsis neglecta 次级代谢产物 抗菌活性 

分 类 号：Q935[生物学—微生物学]