CC10与小鼠心肌梗死后心肌纤维化的关系  

The relationship between CC10 and myocardial fibrosis after myocardial infarction in mice

作　　者：黄挺[1] 沈竹夏 段云娇 刘佳榛 冯京 孙育民[1] 王骏[1] Huang Ting;Shen Zhuxia;Duan Yunjiao;Liu Jiazhen;Feng Jing;Sun Yumin;Wang Jun(Department of Cardiology,Jing􀆳an District Central Hospital,Fudan University,Shanghai,200040,P.R.China)

机构地区：[1]复旦大学附属静安区中心医院心内科,上海200040

出　　处：《老年医学与保健》2022年第2期252-256,262,共6页Geriatrics & Health Care

基　　金：上海市自然科学基金(18ZR1432800,21ZR1455500);上海市卫生和计划生育委员会科研课题(201740176);静安区医学科研课题(2021QN01)。

摘　　要：目的探究克拉拉细胞10-kDa蛋白(CC10)与心肌梗死后心肌纤维化的关系。方法取6~8周龄SPF级雄性C57BL/6小鼠32只,随机分为模型组与假手术组,每组16只。模型组小鼠结扎冠状动脉左前降支,构建心肌梗死模型。检测小鼠心脏的CC10 mRNA、α-SMA mRNA表达及肺内的CC10 mRNA表达。观察小鼠心肌纤维化程度;分析CC10在组织层面和蛋白表达的变化;明确CC10在心脏梗死区定位的细胞类型;测定CC10 mRNA随心脏成纤维细胞增殖的表达变化。结果心肌梗死后,随着心肌纤维化加重,CC10在心脏与肺组织中的表达上升(P<0.05)。CC10的表达主要定位于心脏梗死区的成纤维细胞,且伴随成纤维细胞增殖,CC10的表达不断增加。结论伴随心肌纤维化程度加重,CC10在小鼠心肌梗死后的心脏与肺组织中表达上升。Objective To investigate the relationship between Clara cell 10-kDa protein(CC10)and myocardial fibrosis after myocardial infarction.Methods Thirty two SPF grade male C57 BL/6 mice aged 6-8 weeks were randomly divided into model group and sham operation group,with 16 mice in each group.Mice in the model group were ligated the left anterior descending coronary artery to construct a myocardial infarction model.The expression levels of CC10 mRNA andα-SMA mRNA in heart tissue and CC10 mRNA in the lung tissue were detected.The extent of myocardial fibrosis in mice was observed and the changes of CC10 in tissue level and protein expression were analyzed.The cell types of CC10 located in the infarcted area of the heart were determined;the expression change of CC10 mRNA with the proliferation of cardiac fibroblasts was observed.Results After myocardial infarction,the expression level of CC10 in heart and lung tissues increased with the aggravation of myocardial fibrosis(P<0.05).The expression of CC10 was mainly localized in fibroblasts in the infarcted area of the heart,and the expression of CC10 increased with fibroblast proliferation.Conclusion With the aggravation of myocardial fibrosis,the expression level of CC10 increased in the heart and lung of mice after myocardial infarction.

关 键 词：克拉拉细胞10-kDa蛋白 心肌梗死 心肌纤维化 成纤维细胞 

分 类 号：R542.22[医药卫生—心血管疾病]