云南省某养鸡场鼻气管鸟杆菌核酸检测及其16S rDNA基因序列分析  

Nucleic Acid Detection and 16S rDNA Gene Sequence Analysis of Ornithobacterium rhinotracheale in a Chicken Farm in Yunnan Provinve

作　　者：胡骑[1] 张振兴 宋建领[1] Hu Qi;Zhang Zhenxing;Song Jianling(Yunnan Academy of Animal Husbandry and Veterinary Sciences,Yunnan Key Laboratory of Tropical and Subtropical Animal Viral Diseases,Kunming,Yunnan 650224,China)

机构地区：[1]云南省畜牧兽医科学院,云南省热带亚热带动物病毒病重点实验室,云南昆明650224

出　　处：《中国动物检疫》2022年第6期44-47,共4页China Animal Health Inspection

基　　金：云南省畜禽种质创新及标准化养殖技术推广专项(云财农[2022]5号)。

摘　　要：为确定云南省易门县某养鸡场大量肉鸡出现流鼻涕、打喷嚏、面部肿胀、食欲下降的原因,采集病死肉鸡鼻腔黏液及肺、气管等组织,处理后提取病原核酸,通过RT-PCR/PCR方法分别检测禽流感病毒(AIV)、传染性喉气管炎病毒(ILTV)、鼻气管鸟杆菌(ORT)、鸡副嗜血杆菌(ICV)。结果显示,仅ORT为核酸阳性,其他病原均为阴性。对检出的ORT阳性样品16S rDNA基因进行序列分析发现,阳性样品与伊朗分离株和土耳其/匈牙利分离株的同源性均在99.7%以上,都处于同一分支内,与国内2014年的报道一致,说明云南省流行的ORT变异较少,仅具有相对短的进化史。本研究对于指导该地区鸡群细菌病防控具有参考意义。In order to determine the causes of runny nose,sneezing,facial swelling and loss of appetite in a large number of broiler chickens in a farm in Yimen County,Yunnan Province,tissues such as nasal mucus,lungs and trachea of dead broiler chickens were collected to extract the nucleic acid of the pathogen after disposal,then to detect avian influenza virus(AIV),infectious laryngotracheitis virus(ILTV),Ornithobacterium rhinotracheale(ORT)and Haemophilus paragallinium(ICV),respectively by RT-PCR/PCR.The results showed that positive nucleic acid was observed only in ORT,and negative in all other pathogens.The sequence of 16S rDNA gene of the detected ORT positive samples was analyzed,and it was found that the 16S rDNA gene had more than 99.7%homology with the Iranian and Turkey/Hungary isolates,all of which were located in the same branch,which was similar to the information reported in China in 2014,indicating fewer pathogenic variations of ORT prevalent in Yunnan Province and a relatively short evolutionary history.Therefore,the study was of reference significance to guide the prevention and control of bacterial diseases in chicken flocks in the region.

关 键 词：鼻气管鸟杆菌 检测 序列分析 同源性 

分 类 号：S851.3[农业科学—预防兽医学]