云南省江城县山羊地方性鼻内肿瘤病毒检测及序列分析  

Detection and Sequence Analysis of Goat Enzootic Nasal Tumor Virus in Jiangcheng County,Yunnan Province

作　　者：张娜西 孟锦昕 李钊 何于雯 王德琼 李楠 孙建美 白方 王静林 Zhang Naxi;Meng Jinxin;Li Zhao;He Yuwen;Wang Deqiong;Li Nan;Sun Jianmei;Bai Fang;Wang Jinglin(Jiangcheng Center for Animal Disease Control and Prevention,Jiangcheng,Yunnan 665900,China;Yunnan Tropical and Subtropical Animal Viral Disease Laboratory,Yunnan Animal Science and Veterinary Institute,Kunming,Yunnan 650224,China;Jiangcheng Bureau of Agriculture,Rural Areas and Science and Technology,Jiangcheng,Yunnan 665900,China)

机构地区：[1]江城县动物疫病预防控制中心,云南江城665900 [2]云南省畜牧兽医科学院,云南省热带亚热带动物病毒重点实验室,云南昆明650224 [3]江城县农业农村和科学技术局,云南江城665900

出　　处：《中国动物检疫》2022年第6期48-52,共5页China Animal Health Inspection

基　　金：云南省科技厅重点基金项目(2019FA015,202101AS070219);云南省程功专家工作站项目(202005AF150034);云南省科技人才和平台计划项目(2018HB046);科技创新基地建设项目(202207AB110006)。

摘　　要：为了解云南省山羊群中的地方性鼻内肿瘤病毒(enzootic nasal tumor virus,ENTV)感染情况,2017年在云南省南部边境地区江城县采集山羊血分离血浆,采用随机PCR(random PCR)进行扩增、克隆,结果从20份血浆中获得与ENTV 2型(ENTV-2)同源性较高的2条序列;根据ENTV-2 CHN1(KU258870)毒株序列设计一套半巢式引物,对采集的20份血浆进行核酸检测,结果4份为阳性。序列分析发现,检测到的4条序列与四川、福建等地流行的ENTV-2位于同一个进化分支,它们之间的核苷酸同源性为96.3%~99.5%,氨基酸同源性为95.3%~99.0%,提示江城县羊群中流行的ENV-2可能与四川省、福建省流行毒株相关;位点分析结果显示,江城县的这4份ENTV-2核酸阳性样品在env蛋白186和204位发生了独特的“G→E”氨基酸位点突变,其中1份在178位还发生了“T→P”的氨基酸位点突变。结果表明,江城县山羊群中存在ENTV-2感染,且流行的ENTV-2呈现一些特有的分子特征。因此,云南省需要加强ENTV监测,了解其在山羊群中的流行情况及其演变规律。本研究为进一步开展ENTV感染调查和山羊呼吸道疾病的诊断和预防提供了依据。In order to investigate the infection of enzootic nasal tumor virus(ENTV)in goats in Yunnan Province,goat blood was collected in 2017 to isolate plasma in Jiangcheng County,the southern border area of Yunnan Province,followed by amplification by random PCR and clone,then two sequences with high homology with ENTV-2 were obtained from 20 plasma samples;a set of semi-nested primers were designed based on the sequence of ENTV-2 CHN1(KU258870)to detect the nucleic acid of 20 plasma samples,in which four were positive.It was found that,by sequence analysis,the four sequences were located in the same evolutionary clade as ENTV-2 prevalent in Sichuan and Fujian provinces,with nucleotide homology of 96.3%to 99.5%and amino acid homology of 95.3%to 99.0%,which indicated that ENTV-2 prevalent in goats in the county might be related to those in Sichuan and Fujian provinces;by site analysis,a unique“G→E”amino acid mutation was observed in the four strains when they were located at the positions of 186 and 204 of env protein,and“T→P”in one strain at the position of 178.In conclusion,goats in the county were infected with ENTV-2 that showed some unique molecular characteristics.Therefore,ENTV should be strictly monitored to identify its prevalence and rule of evolution in goats in the province,and then to provide a basis for further efforts against the disease.

关 键 词：地方性鼻内肿瘤病毒 山羊 ENV基因 序列分析 

分 类 号：S852.65[农业科学—基础兽医学]