高效液相色谱法测定尿毒清颗粒中3种成分  

Determination of three constituents in Niaoduqing Particles by high performance liquid chromatography

作　　者：赵磊 李琼[1] ZHAO Lei;LI Qiong(Department of Pharmacy,Wuhu Hospital of Traditional Chinese Medicine,Anhui Province,Wuhu241000,China)

机构地区：[1]安徽省芜湖市中医医院药剂科,安徽芜湖241000

出　　处：《中国医药导报》2022年第12期112-115,共4页China Medical Herald

基　　金：安徽省重点研究与开发计划项目(202104j07020014)。

摘　　要：目的建立同时测定尿毒清颗粒中3种有效成分(芍药苷、二苯乙烯苷、丹酚酸B)的方法。方法采用安捷伦1200高效液相色谱仪测定尿毒清颗粒中3种有效成分,色谱柱为Agilent-ZORBAX SB-C_(18)柱(4.6 mm×250 mm,5μm);流动相为乙腈-0.1%磷酸水,梯度洗脱;流速为1.0 ml/min;检测波长为230、320 nm。结果芍药苷、二苯乙烯苷、丹酚酸B在24.96~249.61μg/ml、12.38~123.76μg/ml、24.84~248.42μg/ml线性关系良好;平均加样回收率分别为100.21%、100.62%、99.87%。结论该实验方法能有效便捷测定3个指标成分的含量,重复性好,为尿毒清颗粒的质量控制及临床疗效的探讨研究提供一定理论基础。Objective To establish a method for simultaneous determination of three active components(paeoniflorin,stilbene glycoside,salvianolic acid B)in Niaoduqing Particles.Methods Agilent 1200 high performance liquid chromatograph was used to determine three active components in Niaoduqing Particles.Chromatographic column was Agilent-ZORBAX SB-C_(18)(4.6 mm×250 mm,5μm);the mobile phase consisted of acetonitrile-0.1%phosphoric acid water with gradient elution;the flow rate was 1.0 ml/min;the detection wavelength was 230,320 nm.Results The linear relationships of paeoniflorin,stilbene glycoside,and salvianolic acid B were 24.96-249.61μg/ml,12.38-123.76μg/ml,24.84-248.42μg/ml;the average recovery rate were 100.21%,100.62%,and 99.87%,respectively.Conclusion The experimental method can effectively and conveniently determine the content of three index components,with good reproducibility,and provide a theoretical basis for the quality control and clinical efficacy of Niaoduqing Particles.

关 键 词：尿毒清颗粒 芍药苷 二苯乙烯苷 丹酚酸B 高效液相色谱法 

分 类 号：R927.1[医药卫生—药学]