On-off-on fluorescence detection for biomolecules by a fluorescent cage through host-guest complexation in water  

作　　者：Honghong Duan Fan Cao Minjie Zhang Meng Gao Liping Cao 

机构地区：[1]College of Chemistry and Materials Science,Northwest University,Xi’an 710069,China [2]National Engineering Research Center for Tissue Restoration and Reconstruction,Key Laboratory of Biomedical Engineering of Guangdong Province,Key Laboratory of Biomedical Materials and Engineering of the Ministry of Education,Innovation Center for Tissue Restoration and Reconstruction,South China University of Technology,Guangzhou 510006,China

出　　处：《Chinese Chemical Letters》2022年第5期2459-2463,共5页中国化学快报（英文版）

基　　金：supported by the National Natural Science Foun-dation of China(Nos.22122108,21971208 and 21771145);the Natu-ral Science Basic Research Plan for Distinguished Young Scholars in Shaanxi Province of China(No.2021JC-37);the Fok Ying Tong Education Foundation(No.171010).

摘　　要：Detection of nucleoside derivatives has paramount importance because they are the essential biomolec-ular units for all life.Herein,we report a host-guest approach by using a fluorescent tetraphenylethene-based octacationic cage as host and 8-hydroxypyrene-1,3,6-trisulfonic acid trisodium salt(HPTS)as guest and fluorescent indicator to form non-fluorescent 1:1:1 host-(endo-exo)guest complex in water.This new host-(endo-exo)guest complex can be successfully used for detecting nucleosides(e.g.,ATP and GTP),DNA(e.g.,sm-DNA),and antibiotics(e.g.,Penicillin G)with off-on fluorescence response via a competitive host-guest exchange with HPTS as exo-guest in water.Furthermore,this on-off-on fluorescent host-guest com-plex is also used for cell imaging based on ATP concentration in HeLa cells.Therefore,this study not only provides insight into the construction of a supramolecular probe with on-off-on fluorescence via host-guest complexation and exchange in solution,but also realizes a universal method for detecting and monitoring biomolecules.

关 键 词：Host-guest complexation Tetraphenylethene-based octacationic cage Nucleoside derivatives FLUORESCENCE Cell imaging 

分 类 号：O641.4[理学—物理化学] O657.3[理学—化学] Q503[生物学—生物化学]