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作 者:Yixin Liu Yuanyuan Yao Beibei Yang Yan-Jun Liu Baohong Liu
机构地区:[1]Department of Chemistry,Shanghai Stomatological Hospital,and State Key Lab of Molecular Engineering of Polymers,Fudan University,Shanghai 200433,China [2]Shanghai Key Laboratory of Medical Epigenetics,International Co-laboratory of Medical Epigenetics and Metabolism(Ministry of Science and Technology),Institutes of Biomedical Sciences,Fudan University,Shanghai 200032,China [3]College of Biological,Chemical Sciences and Engineering,Jiaxing University,Jiaxing 314001,China [4]Department of Chemistry and Chemical Engineering,Nantong University,Nantong 226000,China
出 处:《Chinese Chemical Letters》2022年第5期2705-2707,共3页中国化学快报(英文版)
基 金:supported by the National Natural Science Foundation of China(Nos.21934001 and 31870978);the Natu-ral Science Foundation of Zhejiang Province(No.LQ20B050002).
摘 要:Cell migration proceeds in 3D matrices in vivo,which can naturally switch to distinct phenotypes for bet-ter invasion in confined microenvironments.The studies of important metabolites under confinement are extremely meaningful for comprehensive insights into cancer metastasis.The integration of cell confine-ment device and analytical techniques is a key point for in-situ analysis of significant metabolites in vitro.Herein,an electrochemiluminescence(ECL)sensing platform was designed for in-situ monitoring of cell-secreted lactate in highly confined microenvironments.The 3-μm confiner was exactly fabricated via mi-crofabrication and microfluidics technique,and cells in high confinement and low adhesion tended to be round with contractile blebs on cell margins.Significantly,in-situ monitoring of lactate was successfully achieved on the ECL platform with the catalysis of lactate oxidase,in which the levels in different time intervals were acquired in the luminol-hydrogen peroxide system.Furthermore,the results were verified by the liquid chromatography-tandem mass spectrometry(LC-MS/MS)technology,which showed similar fluctuations with the ECL platform.This system offered an available avenue for metabolites analysis in highly confined microenvironments,which may advance deeper insights into metabolic mechanisms of cancer metastasis.
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