小麦KNOX基因家族鉴定与生物信息学分析  

Identification and Bioinformatics Analysis of KNOX Gene Family in Wheat(Triticum aestivum L.)

作　　者：宋宁宁 梁慧慧 安义伟 柏胜龙 麻菲菲 张震 李浩 周云[1] 郭光辉 宋纯鹏[1] Song Ningning;Liang Huihui;An Yiwei;Bai Shenglong;Ma Feifei;Zhang Zhen;Li Hao;Zhou Yun;Guo Guanghui;Song Chunpeng(State Key Laboratory of Crop Stress Adaptation and Improvement,School of Life Sciences,Henan University,KaiFeng,475004)

机构地区：[1]河南大学生命科学学院省部共建作物逆境适应与改良国家重点实验室,开封475004

出　　处：《分子植物育种》2022年第7期2105-2117,共13页Molecular Plant Breeding

基　　金：国家自然科学基金项目(31901547);中国博士后基金项目(2018M642741);河南省博士后科研项目(001801-013)共同资助。

摘　　要：KNOX基因家族是编码同源异型盒蛋白的转录因子,在植物生长发育和形态建成过程中起着重要的调控作用。然而,关于小麦KNOX基因家族的研究很少。本研究利用生物信息学手段从小麦基因组中鉴定到分布于18条染色体上的36个KNOX基因,所有鉴定到的家族成员均含有KNOX1或KNOX2结构域。本研究对KNOX基因家族的系统进化、基因结构、蛋白结构域、顺式作用元件和基因表达模式进行了分析。根据其系统发育关系,将小麦KNOX基因分为ClassⅠ和ClassⅡ两大亚类,两亚类又进一步分为5个进化分支。大多数小麦KNOX基因含有典型KNOX1、KNOX2、ELK、HOX四个蛋白保守结构域。小麦KNOX基因的启动子分析发现了一些与激素、生长发育和胁迫响应相关的顺式作用调控元件。不同组织器官转录组数据分析显示,ClassⅠ类KNOX基因具有明显的组织特异性,ClassⅡ类KNOX基因的表达部位更广泛。本研究为进一步分析小麦KNOX基因家族的功能和调控机制提供了重要信息。KNOTTED-like homeodomain(KNOX)gene family is a transcription factor encoding homeobox protein,which plays an important role in plant growth and morphogenesis.However,little information is available on the KNOX gene family in wheat(Triticum aestivum L.).In this study,36 KNOX genes with KNOX1 or KNOX2domain distributed on 18 chromosomes were identified from wheat genome using bioinformatics methods.The phylogenetic evolution,gene structure,protein domains,cis-acting elements and gene expression patterns were analyzed in the present study.Based on the phylogenetic tree,the 36 TaKNOX genes were divided into two major subclasses,ClassⅠand ClassⅡ,and the two subclasses were further divided into five evolutionary branches.Most TaKNOX genes contained four typical conserved protein domains:KNOX1,KNOX2,ELK and HOX.Some cis-acting elements associated with hormonal,plant development and stress in TaKNOX promoters.The analysis result of transcriptome data from wheat different tissue showed that ClassⅠKNOX genes had obvious tissue specificity,while ClassⅡKNOX genes expressed widely in different wheat tissues.The study results provide important information for future analysis of the regulation and functions of the TaKNOX gene family.

关 键 词：小麦 KNOX基因家族 基因结构 基因表达 

分 类 号：S512.1[农业科学—作物学]