寒兰FLOWERING LOCUS T基因克隆及周期性表达水平分析  

Isolation of FL OWERING LOCUS T Gene and Analysis of Periodic Expression Level in Cymbidium kanran

作　　者：应震 周庄[1] 杨燕萍[1] 付双彬 徐婉 Ying Zhen;Zhou Zhuang;Yang Yanping;Fu Shuangbin;Xu Wan(Zhejiang Institute of Subtropical Crops,Wenzhou,325005)

机构地区：[1]浙江省亚热带作物研究所,温州325005

出　　处：《分子植物育种》2022年第7期2135-2142,共8页Molecular Plant Breeding

基　　金：浙江省自然科学基金项目(LY19C150003);温州农业新品种选育协作组项目(2019ZX004-3);浙江省农科院青年科技人才培养项目(2019R26R08E01)共同资助。

摘　　要：寒兰(Cymbidium kanran)为中国五大国兰之一,主要花期10~12月,具有极高的观赏价值。为研究寒兰花期调控,本研究对寒兰FT基因序列进行扩增,并对其完整花期的组织特异性表达进行分析。结果表明:(1)寒兰FT基因ORF序列长度为531 bp,编码177个氨基酸,序列与已报到的建兰、春兰等兰属植物高度同源;(2)在8和9月期间,开花寒兰叶片中的FT基因表达水平均比未开花的寒兰高1.3倍,其余时间该基因表达水平差异不显著;(3)在8至12月期间,开花寒兰假鳞茎中的FT基因表达水平分别是未开花寒兰的1.56、2.46、3.10、2.70和1.42倍;(4)在花期中,假鳞茎FT基因相对表达水平高于叶片至少15.80倍;(5)假鳞茎中FT基因表达水平高于花序梗和花被片,略低于初生花芽。上述结果充分说明自然开花的寒兰叶片与假鳞茎的FT基因在特定时期的表达模式有重要的相关性,这为今后进行寒兰花期调控研究和人工催花试验都具有理论意义和应用价值。Cymbidium kanran,as one of the five most famous Chinese cymbidium,has high ornamental value,and it’s principal flowering period varies from October to December.In order to study the flowering-regulation,the FLOWERING LOCUS T gene(FT)of C.kanran sequence was amplified,and tissue-specific expression of the gene was analyzed in the whole flowering period.The results show:(1)The ORF sequence of the FT is 531 bp in length and the gene encodes 177 amino acids,and the sequence is highly homologous to the reported cymbidium such as C.ensifolium and C.goeringii;(2)During August to September,the expression of FT in the leaves of flowering C.kanran was 1.3 times higher than that of unflowered group,but there was no significant difference in the expression level of this gene at the other months;(3)During August to December,the expression level of FT in the flow ering C.kanran pseudobulbs were 1.56,2.46,3.10,2.70,and 1.42 times than that of unflowered group,respectively;(4)During flowering,the relative expression level of FT in pseudobulb of flowering C.kanran was at least 15.80times higher than that in leaves;(5)The expression of FT in pseudobulbs were higher than that of flower stem and perianths,and slightly lower than that of primary flower bud.The above results fully show that there is an important correlation between the expression pattern of FT of the leaves and pseudobulbs in a specific period,which provides theoretical significance and application value for future research in the flowering-regulation of C.kanran and artificial flowering experiments.

关 键 词：寒兰 花期 FT基因 表达模式 

分 类 号：S682.31[农业科学—观赏园艺]