1-MCP处理对不同肉质桃果实糖、酸含量及相关基因表达水平的影响  

Effects of 1-MCP Treatment on Sugar Acid Content and Related Genes Expression in Different Flesh Texture Peaches

作　　者：谢斯雯 桑倩姿 姜航 孟静璇 罗东涛 陈亚辉[2] 张竹茂 宋志忠 Xie Siwen;Sang Qianzi;Jiang Hang;Meng Jingxuan;Luo Dongtao;Chen Yahui;Zhang Zhumao;Song Zhizhong(Key Laboratory of Molecular Module-Based Breeding of High Yield and Abiotic Resistant Plants in Universities of Shandong,College of Agriculture,Ludong University;College of Forest,Nanjing Forest University;Longkou Campus of Shandong Agricultural Radio and Television School)

机构地区：[1]鲁东大学农学院山东省高等学校重点实验室作物高产抗逆分子模块育种实验室,烟台264025 [2]南京林业大学林学,南京214000 [3]山东省农业广播电视学校龙口市分校,龙口265701

出　　处：《分子植物育种》2022年第7期2170-2178,共9页Molecular Plant Breeding

基　　金：国家重点研究计划子课题(2019YFD1000500);山东省农业良种工程项目(2019LZGC009);山东省重点研发计划项目(2018JHZ006)共同资助。

摘　　要：以软溶质‘红清水’、硬溶质‘寒露蜜’、不溶质‘阿部白桃’和硬质型(stony hard,SH)‘有名白桃’4个不同肉质桃品种为试材,研究1-甲基环丙烯(1-methylcyclopropene,1-MCP)处理对果实采后贮藏中主要可溶性糖和有机酸代谢及其合成相关基因表达的影响。结果表明,SS基因表达水平与软溶质桃蔗糖代谢呈显著正相关性,1-MCP处理通过调控SS基因表达,显著维持了软溶质和不溶质桃蔗糖含量的稳定。PEPC基因表达水平与硬溶质、硬质型桃苹果酸代谢呈现显著正相关性,1-MCP处理通过提高PEPC基因表达水来增加硬溶质桃苹果酸的含量。ME基因表达水平与不溶质桃苹果酸含量具有显著负相关性,1-MCP处理则抑制了ME基因表达水平,进而提高了苹果酸的含量。In this study,four different peach cultivars of’Hongqingshui’with soft-melting,’Hanlumi’with hardmelting,’Abubaitao’with non-melting,and’Youmingbaitao’with stonyhard were collected as test materials.The effects of 1-methylcyclopropene(1-MCP)application on sugar metabolism and acid metabolism,and expression profiles of related genes in postharvest peach fruits were studied.Results showed that the expression level of SS gene was positively correlated with sugar metabolism in peach cultivars with soft-melting,and the contents of sucrose in peach cultivars with soft-melting and non-melting were maintained(non-significantly changed)by 1-MCP treatment.The expression level of PEPC gene was positively correlated with the sucrose metabolism of peach cultivars with hard-melting and stonyhard,and 1-MCP treatment increased peach malic acid content by inducing PEPC gene expression.There was a negative correlation between ME gene expression and malic acid content in peach cultivars with non-melting,and 1-MCP treatment inhibited the expression of ME gene and then increased the malic acid content.

关 键 词：1-甲基环丙烯(1-MCP) 桃 苹果酸 蔗糖 转录调控 

分 类 号：S662.1[农业科学—果树学]