新疆扁桃自交不亲和PsdSSK1基因载体构建及转化矮牵牛分析  

Analysis of Self-incompatibility PsdSSK1 Gene Vector Construction in Xinjiang Almond and Transformation into Petunia

作　　者：唐义莲 余镇藩 曾斌[1] 张冬冬 阿布都卡尤木·阿依麦提 刘泉鑫 岳婉婉 杨佳惠 高雯雯 Tang Yilian;Yu Zhenfan;Zeng Bin;Zhang Dongdong;Abu Du Kayoumu·Ayimati;Liu Quanxin;Yue Wanwan;Yang Jiahui;Gao Wenwen(Xinjiang Sub-branch of National Melon and Fruit Improvement Center,College of Forestry and Horticulture,Xinjiang Agricultural University,Urumqi,830052)

机构地区：[1]新疆农业大学林学与园艺学院国家瓜果改良中心新疆分中心,乌鲁木齐830052

出　　处：《分子植物育种》2022年第7期2294-2301,共8页Molecular Plant Breeding

基　　金：新疆维吾尔自治区自然科学基金项目(2019D01A56)资助。

摘　　要：为探索适合新疆扁桃自交不亲和PsdSSK1基因的根癌农杆菌介导的矮牵牛遗传转化体系,本研究构建了扁桃PsdSSK1基因的表达载体pCAMBIA1301-35S-NOS-PsdSSK1,并通过农杆菌介导法将其转入矮牵牛,在前期进行培养基激素浓度筛选的基础上再进行抗生素浓度的筛选。结果表明:不定芽诱导培养基最佳配方为MS+2.0 mg/L 6-BA+0.2 mg/L NAA,矮牵牛出芽率为100%;不定根诱导培养基最佳配方为1/2MS+0.1 mg/L IBA,矮牵牛生根率为96.67%;抗生素Kan和Cef的最佳使用浓度分别为75和400 mg/L。获得了转PsdSSK1基因的矮牵牛阳性植株。本实验构建了PsdSSK1基因的表达载体,并成功筛选出一套适合农杆菌介导的矮牵牛遗传转化体系,为今后研究PsdSSK1基因的生物学功能提供参考。In order to explore a petunia genetic transformation system mediated by Agrobacterium tumefaciens suitable for self-incompatibility PsdSSK1 gene of Amygdalus communis L.in Xinjiang.In this study,the expression vector p CAMBIA1301-35S-NOS-PsdSSK1 of Prunus amygdalus was constructed and transferred to petunia with the Agrobacterium-mediated method,and then the antibiotic concentration was screened on the basis of the preliminary screening of hormone concentration in culture medium.The results showed that the optimal formulation of adventitious bud induction medium was MS+2.0 mg/L 6-BA+0.2 mg/L NAA,and the germination rate of petunia was 100%.The optimal formulation of adventitious root induction medium was 1/2 MS+0.1 mg/L IBA,and the rooting rate of Petunia was 96.67%.The optimal concentrations of antibiotics Kan and Cef were 75 mg/L and 400 mg/L,respectively.Moreover,the petunia positive plants with PsdSSK1 gene were obtained.In the current experiment,the expression vector of PsdSSK1 gene was constructed,and a set of genetic transformation system suitable for Agrobacterium-mediated petunia were successfully screened out,so as to provide information for future research on the biological function of PsdSSK1 gene.

关 键 词：扁桃 Psd SSK1基因 农杆菌介导法 矮牵牛 

分 类 号：S662.9[农业科学—果树学]