利用分子标记鉴定优质籼稻粒形相关基因的基因型  

Genotyping of Grain Shape Related Genes in High Quality Xian/indica Varieties by Molecular Marker

作　　者：周雷[1] 李二敬 徐华山[1] 陈志军[1] 杨国才[1] 刘凯[1] 游艾青[1,3] Zhou Lei;Li Erjing;Xu Huashan;Chen Zhijue;Yang Guocai;Liu Kai;You Aiqing(Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement,Food Crops Institute,Hubei Academy of Agricultural Sciences,Wuhan,430064;College of Life Sciences,Wuhan University,Wuhan,430072;Hubei Collaborative Innovation Center for Grain Industry,Yangtze University,Jingzhou,434025)

机构地区：[1]湖北省农业科学院粮食作物研究所粮食作物种质创新与遗传改良湖北省重点实验室,武汉430064 [2]武汉大学研究生院,武汉430072 [3]长江大学主要粮食作物产业化湖北省协同创新中心,荆州434025

出　　处：《分子植物育种》2022年第7期2326-2334,共9页Molecular Plant Breeding

基　　金：“十三五”国家重点研发计划项目(2018YFD0301305,2017YFD0100305);湖北省重点研发项目(2020BBB051);国家水稻产业技术体系武汉综合试验站项目(CARS-01-78)共同资助。

摘　　要：本研究选取12个优质籼稻品种为供试材料,利用分子标记检测方法,对这些品种中的7个粒长基因GS3、LGY3、q GL3、GL7、SLG7、TGW6、GS9,和3个粒宽基因GW8、GW5、GS5进行了基因型分析,结果发现:12个籼稻品种都具有gs3长粒等位基因,‘鄂中5号’、‘玉晶91’、‘玉针香’、‘黄毛占’、‘洋西早’、‘超级巴斯巴蒂’具有GL7和SLG7长粒等位基因,除‘鉴真2号’外其他11个品种都具有gw8细长粒等位基因,‘玉晶91’、‘玉针香’、‘9311’、‘R835’、‘黄毛占’、‘洋西早’具有GS5宽粒等位基因。本研究不仅对现有优质籼稻品种粒型基因型有一个较全面的认识,还为今后利用分子标记辅助培育长粒籼稻新品种和亲本选择提供重要的基因型信息。12 rep resentative high quality xian/indica rice varieties were selected as test materials.Seven grain length-related genes including GS3,LGY3,qGL3,GL7,SLG7,TGW6 and GS9,and three grain width-related genes including GW8,GW5 and GS5 were tested by molecular marker.The results showed that:All 12 varieties have the gs3long-grain allele,’Ezhong 5’,’Yujing91’,’Yuzhenxiang’,’Huangmaozhan’,’Yangxizao’and’Super Basimati’have the GL7 and SLG7 long-grain alleles,11 varieties with the exception of Jianzhen 2 have the gw8 slender-grain allele,’Yujing 91’,’Yuzhenxiang’,’9311’,’R835’,’Huangmaozhan’,’Yangxizao’have the GS5 wide-grain allele.This study is helpful to establish an overall understanding of the genotypes of grain-shape genes in high quality xian/indica varieties,and provides important genotype information in selecting and cultivating new high quality long-grain indica varieties via molecular marker-assisted selection in future.

关 键 词：优质籼稻 粒型基因 分子标记 基因型 

分 类 号：S511.21[农业科学—作物学]