萝卜LIM基因家族生物信息学及表达分析  

Bioinformatics and Expression Analysis of LIM Gene Family in Radish(Raphanus sativus)

作　　者：孙继峰[1] 宋倩 徐立功[1] 周陆红 谭金霞 李萌[1] 杨晓东[1] 宋银行 韩太利 Sun Jifeng;Song Qian;Xu Ligong;Zhou Luhong;Tan Jinxia;Li Meng;Yang Xiaodong;Song Yinhang;Han Taili(Weifang Academy of Agricultural Science in Shandong Province,Weifang,261017;Ganzhou Academy of Science in Jiangxi Province,Ganzhou,341000)

机构地区：[1]山东省潍坊市农业科学院,淮坊261017 [2]江西省赣南科学院,赣州341000

出　　处：《分子植物育种》2022年第8期2485-2494,共10页Molecular Plant Breeding

基　　金：国家大宗蔬菜产业技术体系建设专项(GARS-23-G13);山东省农业良种工程项目(2017LZN044,2019LZGC006)共同资助。

摘　　要：为明确LIM家族基因在萝卜小孢子胚胎发生诱导中的表达特性,本研究在萝卜全基因组中鉴定LIM,通过分析理化性质、基因及蛋白结构、基因进化和表达模式。共鉴定到12个萝卜LIM家族成员,包含189~320个不等的氨基酸。亚细胞定位结果显示,RsLIM蛋白在细胞核中均有分布,部分在高尔基体中也有分布。启动子顺式作用元件分析表明,RsLIM启动子回应光、激素诱导的元件最多。基因结构分析表明,RsLIM中外显子数为3~10个。结构域分析表明,RsLIM蛋白中除Rs LIM2外,均具有2个LIM结构域。根据聚类分析结果可将RsLIM分为3组。染色体定位结果显示,Rs LIM分布在萝卜9条染色体中的6条,且呈不均匀分布。转录组数据表明,12个RsLIM基因在不同组织中的表达具有明显差异,显著分为2类。游离小孢子不同预处理的实时荧光定量PCR结果显示,12个Rs LIM对不同预处理的反应效果不同,低温诱导促进多数基因表达,高温诱导抑制多数基因的表达,RsLIM基因家族具有一定的表达特异性。本研究为萝卜LIM基因家族的功能分析提供了依据。In order to clarify the expression characteristics of LIM gene family in the induction of radish microspore embryogenesis,this study were carried out to analyze the bioinformatics(e.g physical and chemical properties,gene and protein structure,gene evolution)and expression of the LIM gene family in radish.12members of the LIM family of radish were identified.These RsLIM proteins contained 189~320 amino acids.The results of subcellular localization showed that the Rs LIM protein was distributed in the nucleus.The results of cis acting element analysis of the promoter showed that RsLIM promoter had the most response to light and hormone induction.The results of gene structure analysis showed that there were 3~10 exons in Rs LIM proteins.The domain analysis showed that RsLIM protein had 2 LIM domains except Rs LIM2.According to the results of cluster analysis,Rs LIM can be divided into three groups.The results of chromosome mapping showed that RsLIM was distributed in 6 of 9 chromosomes,and the distribution was uneven.The transcriptome data showed that there were significant differences in the expression of 12 Rs LIM genes in different tissues.The results of real-time quantitative PCR showed that 12 RsLIM had different effects on different pretreatments.The low temperature promoted the expression of most genes,while the high temperature inhibited the expression of most genes.This study provides a basis for functional analysis of LIM gene family in radish.

关 键 词：萝卜 LIM基因家族 生物信息学 表达分析 

分 类 号：S631.1[农业科学—蔬菜学]