植物蔗糖磷酸合成酶(SPS)基因研究进展  

Research Progress of Sucrose Phosphate Synthetase(SPS)Gene in Plants

作　　者：吴建阳 陈妹[3] 姚艳丽[3] 张秀梅[3] Wu Jianyang;Chen Mei;Yao Yanli;Zhang Xiumei(Zhanjiang Preschool Education College,Zhanjiang,524037;Basic Education College of Lingnan Normal University,Zhanjiang,524037;Key Laboratory of Tropical Fruit Biology,Ministry of Agriculture&Rural Affairs,South Subtropical Crop Research Institute,Chinese Academy of Tropical Agricultural Science,Zhanjiang,524091)

机构地区：[1]湛江幼儿师范专科学校,湛江524037 [2]岭南师范学院基础教育学院,湛江524037 [3]中国热带农业科学院南亚热带作物研究所农业农村部热带果树生物学重点实验室,湛江524091

出　　处：《分子植物育种》2022年第8期2597-2606,共10页Molecular Plant Breeding

基　　金：广东省现代农业技术体系创新团队建设专项(2022KJ109);广东省普通高校特色创新项目(2020KTSCX350);湛江幼儿师范专科学校科学研究特色创新项目(ZJYZTS202001);广东省科技创新战略专项资金(“攀登计划”专项资金)(pdjh2021b0980)共同资助。

摘　　要：蔗糖磷酸合成酶(SPS,EC 2.4.1.14)是调控蔗糖合成的关键限速酶,SPS受SPS基因家族编码,不同物种中SPS蛋白长度介于468~1107 aa之间,分子量在104.1~120.1 kD之间,理论等电点范围为5.81~6.76,外显子数量介于9~14之间,内含子数量介于10~13之间。进化分析表明SPS基因可以分为A、B、C、D 4个亚家族,同一植物中不同亚家族SPS基因的时空表达具有差异,功能也有所差异,光温条件和栽培措施可以显著影响SPS基因的表达。转SPS基因研究表明其对植物的蔗糖含量、产量、生物量和纤维含量有显著的影响。本研究对SPS基因在国内外的研究现状进行了总结,并展望了未来的研究方向,以期为进一步揭开植物蔗糖代谢的分子调控机制、加快植物分子育种提供理论参考。Sucrose phosphate synthase(SPS,EC 2.4.1.14)is a key enzyme that regulates sucrose synthesis in plants.SPS is encoded by SPS gene family.In different species,the length of SPS protein ranged from 468 to1107 aa,the molecular weight ranged from 104.1 to 120.1 k D,the theoretical isoelectric point ranged from 5.81 to6.76,the number of exons ranged from 9 to 14,and the number of introns ranged from 10 to 13.Evolutionary analysis shows that SPS genes can be divided into 4 subfamilies including A,B,C,and D.Although in the same plant,the transcription of SPS genes in different subfamilies has tissue and organ specificity,and functions are also different.The key SPS gene regulating sucrose metabolism are different in different species.Light and temperature conditions can significantly affect the expression of the SPS gene.Transgenic researches showed that SPS gene had significant effects on sucrose content,plant yield,biomass and fibercontent.In order to provide theoretical reference for further exploring the molecular regulation mechanism of sucrose metabolism and accelerating molecular breeding,this paper summarizes the research status of SPS gene at home and abroad,and looks forward to the future research direction.

关 键 词：蔗糖 蔗糖磷酸合成酶 基因家族 基因表达 

分 类 号：Q943.2[生物学—植物学]