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作 者:魏崃[1] 王伟威[1] 赵贵兴[1] 闫洪睿[1] 毕伟伟[1] 刘丽君[1] Wei Lai;Wang Weiwei;Zhao Guixing;Yan Hongrui;Bi Weiwei;Liu Lijun(Soybean Research Institute of Heilongjiang Academy of Agricultural Sciences,Harbin,150086)
机构地区:[1]黑龙江省农业科学院大豆研究所,哈尔滨150086
出 处:《分子植物育种》2022年第8期2657-2664,共8页Molecular Plant Breeding
基 金:国家大豆产业技术体系项目(CARS-O4-PSO5);抗逆转基因大豆新品种培育项目(2016ZX08004002);农业科技创新跨越工程项目(HNK2019CX01);哈尔滨市科技局项目(2017RAQYJ034);黑龙江省博士后基金项目(LBH-Z16184);黑龙江省农业科学院院级课题项目(2017BZ14)共同资助。
摘 要:为解析东北高寒地区极早熟骨干亲本的分子遗传特性,采用田间表型调查、病虫害接种鉴定和SLAF-seq技术相结合的方法,对黑龙江省高寒地区极早熟骨干亲本及其后代衍生品种的遗传性状和基因组遗传特征进行解析,结果表明:‘黑河54’与衍生品种间遗传距离较近;‘黑河54’与‘黑河18’的遗传距离较近,遗传距离0.416,而与‘北丰11’遗传距离较远,遗传距离0.570;而‘黑河18’与‘黑河9’的遗传距离最近,遗传距离为0.145;‘黑河54’骨干亲本与其衍生品种在具有相同的基因组区段,且在4号、9号染色体上的相同等位变异超过94%以上,结果显示,极早熟组骨干亲本具有早熟、尖叶、抗倒伏、生育期短、油分、结荚习性、低温下的发芽能力、适应性强、高产的特点,这成为骨干亲本在后代的遗传基础。In order to analyze the molecular genetic characteristics of very early mature backbone parents in the northeastern alpine region,the field phenotype survey,identification of plant diseases and insect pests inoculation and SLAF-seq technology were combined to research genomic genetic characteristics of the very early mature backbone parents and their offspring derived varieties in the high cold region of Heilongjiang Province.The results showed that the genetic distance between’Heihe54’and the derived varieties was close;the genetic distance between’Heihe54’and’Heihe18’was close,with a genetic distance of 0.416,however the genetic distance with’Beifeng11’was far,with genetic distance of 0.570;while the genetic distance between’Heihe18’and’Heihe9’is the closest,with a genetic distance of 0.145.The backbone parents of’Heihe54’and their derivatives have the same genomic segment,and the same allelic variation on chromosomes 4 and 9 exceeds 94%.The results showed that the backbone parents of the extremely early maturity group had the characteristics of early maturity,pointed leaves,lodging resistance,short growth period,oil content,pod setting habit,germination ability at low temperature,strong adaptability and high yield,which became the genetic basis of the backbone parents in the offspring.
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