Low-power STED nanoscopy based on temporal and spatial modulation  

作　　者：Luwei Wang Yue Chen Yong Guo Weixin Xie Zhigang Yang Xiaoyu Weng Wei Yan JunleQu 

机构地区：[1]Center for Biomedical Photonics&College of Physics and Optoelectronic Engineering,Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province,Shenzhen 518060,China [2]College of Electronics and Information Engineering,Shenzhen University,Shenzhen 518060,China

出　　处：《Nano Research》2022年第4期3479-3486,共8页纳米研究（英文版）

基　　金：This work has been partially supported by the National Basic Research Program of China(No.2017YFA0700500);the National Natural Science Foundation of China(Nos.61620106016,61835009,62005171,and 61975127);Guangdong Natural Science Foundation(Nos.2019A1515110380 and 2020A1515010679);Shenzhen International Cooperation Project(No.GJHZ20180928161811821);Shenzhen Basic Research Project(No.JCYJ20180305125304883);China Post-doctoral Science Foundation(No.2019M663050).

摘　　要：Stimulated emission depletion(STED)nanoscopy enables the visualization of subcellular organelles in unprecedented detail.However,reducing the power dependency remains one of the greatest challenges for STED imaging in living cells.Here,we propose a new method,called modulated STED,to reduce the demand for depletion power in STED imaging by modulating the information from the temporal and spatial domains.In this approach,an excitation pulse is followed by a depletion pulse with a longer delay;therefore,the fluorescence decay curve contains both confocal and STED photons in a laser pulse period.With time-resolved detection,we can remove residual diffraction-limited signals pixel by pixel from STED photons by taking the weighted difference of the depleted photons.Finally,fluorescence emission in the periphery of an excitation spot is further inhibited through spatial modulation of fluorescent signals,which replaced the increase of the depletion power in conventional STED.We demonstrate that the modulated STED method can achieve a resolution of<100 nm in both fixed and living cells with a depletion power that is dozens of times lower than that of conventional STED,therefore,it is very suitable for long-term superresolution imaging of living cells.Furthermore,the idea of the method could open up a new avenue to the implementation of other experiments,such as light-sheet imaging,multicolor and three-demensional(3D)super-resolution imaging.

关 键 词：fluorescence microscopy super-resolution imaging stimulated emission depletion fluorescence lifetime signal modulation 

分 类 号：TH742[机械工程—光学工程] TB383[机械工程—仪器科学与技术]