水中运动联合中药内治对糖尿病大鼠心肌细胞凋亡和心功能的影响  

Effects of Aquatic Therapeutic Exercise Combined with Traditional Chinese Medicine Internal Treatment on Cardiomyocyte Apoptosis and Cardiac Function in Diabetic Rats

作　　者：黄光明 赵兴昌 符显昭[2] HUANG Guang-ming;ZHAO Xing-chang;FU Xian-zhao(Clinical Medical College,Youjiang Medical University for Nationalities,Baise 533000,Guangxi,China;Department of Traditional Chinese Medicine,the Affiliated Hospital of Youjiang Medical University for Nationalities,Baise 533000,Guangxi,China)

机构地区：[1]右江民族医学院临床医学院,广西百色533000 [2]右江民族医学院附属医院中医科,广西百色533000

出　　处：《医学信息》2022年第11期72-76,共5页Journal of Medical Information

基　　金：国家自然科学基金项目(编号:81960833)。

摘　　要：目的观察水中运动联合中药内治对糖尿病大鼠心肌细胞凋亡和心功能的影响。方法SD雄性大鼠125只,随机均分为空白组、模型组、水中运动组、中药内治组、水中运动联合中药内治组(综合康复组),每组25只。除空白组外,其余组利用链脲佐菌素+高脂饲养构建二型糖尿病大鼠模型。造模成功后,各治疗组接受相应药物与运动方案,8周后,超声心动图检测各组LVEDD、LVESD及EF、E/A;RT-PCR检测各组心肌细胞内质网应激标志物GRP78和Caspase-12的mRNA转录水平;TUNEL法检测各组心肌细胞凋亡水平。结果模型组LVEDD、LVESD水平高于空白组,E/A、EF均低于空白组,差异有统计学意义(P<0.05);模型组GRP78、Caspase-12 mRNA转录水平和心肌细胞凋亡水平高于空白组,统计学意义显著(P<0.01);水中运动组、中药内治组、综合康复组LVEDD、LVESD水平低于模型组,E/A、EF高于模型组(P<0.05);三组GRP78及Caspase-12mRNA转录水平、心肌细胞凋亡水平均低于模型组(P<0.05);综合康复组各项指标改善最为明显,与水中运动组各项指标比较,差异有统计学意义(P<0.05)。结论水中运动联合中药内治可降低心肌细胞凋亡水平,抑制心脏重塑,保护心功能,其机制可能与减轻心肌细胞内质网应激,抑制Caspase-12凋亡通路有关。Objective To observe the effects of aquatic therapeutic exercise combined with traditional chinese medicine internal treatment on cardiomyocyte apoptosis and cardiac function in type 2 diabetic rats.Methods A total of 125 healthy SD male rats were randomly divided into blank group,model group,aquatic therapeutic exercise group,traditional Chinese medicine internal treatment group,aquatic therapeutic exercise group combined traditional Chinese medicine internal treatment group(comprehensive rehabilitation group)with 25 rats in each group.In addition to the blank group,the rat model of type 2 diabetes was established by streptozocin+high fat feeding.After successful modeling,each treatment group received corresponding drugs and exercise programs.After 8 weeks,LVEDD,LVESD,EF and E/A were detected by echocardiography.RT-PCR was used to detect the mRNA transcription levels of endoplasmic reticulum stress markers GRP78 and Caspase-12 in each group.TUNEL method was used to detect the apoptosis level of cardiomyocytes in each group.Results The levels of LVEDD and LVESD in the model group were higher than those in the blank group,and E/A and EF were lower than those in the blank group,with significant difference(P<0.05).The transcription levels of GRP78 and Caspase-12 mRNA and myocardial cell apoptosis in the model group were higher than those in the blank group,and the statistical significance was significant(P<0.01).The levels of LVEDD and LVESD in the aquatic therapeutic exercise group,traditional Chinese medicine internal treatment group and comprehensive rehabilitation group were lower than those in the model group,and the E/A and EF were higher than those in the model group(P<0.05).The levels of GRP78 and Caspase-12 mRNA transcription and cardiomyocyte apoptosis in the three groups were lower than those in the model group(P<0.05).The improvement of each index in the comprehensive rehabilitation group was the most obvious,and the difference was statistically significant compared with that in the water exercise group(

关 键 词：糖尿病 内质网应激 水中运动治疗 中药内治 心脏重塑 康复治疗 心力衰竭 

分 类 号：R542.2[医药卫生—心血管疾病]