HPLC测定不同产区生何首乌和不同炮制工艺制何首乌中蒽醌类成分含量  

Determination of anthraquinones in Polygoni Multiflori Radix from different origins and processed differently by HPLC

作　　者：高慧宇 杨建波[1] 孙华[2] 宋云飞 程显隆[1] 王雪婷 魏锋[1] 汪祺[1] 王莹[1] 靳洪涛[2] 马双成[1] GAO Huiyu;YANG Jianbo;SUN Hua;SONG Yunfei;CHENG Xianlong;WANG Xueting;WEI Feng;WANGQi;WANG Ying;JIN Hongtao;MA Shuangcheng(National Institutes for Food and Drug Control,Beijing 100050,China;Institute of Materia Medica,Chinese Academy of Medical Science,Beijing 100050,China)

机构地区：[1]中国食品药品检定研究院,北京100050 [2]中国医学科学院药物研究所,北京100050

出　　处：《中国药物警戒》2022年第5期522-526,共5页Chinese Journal of Pharmacovigilance

基　　金：国家自然科学基金资助项目(81773874、81973476)。

摘　　要：目的采用高效液相色谱法(HPLC)同时测定不同产地何首乌和不同炮制工艺制何首乌中大黄素8-O-β-D-葡萄糖苷、大黄素、大黄素甲醚、游离蒽醌和结合蒽醌的含量。方法采用Agilent 5 TC-C_(18)(2)色谱柱(250 mm×4.6 mm,5μm),以甲醇-0.1%甲酸水为流动相梯度洗脱,流速1.0 mL·min^(-1),检测波长254 nm,测定大黄素8-O-β-D-葡萄糖苷、大黄素和大黄素甲醚的含量;按照《中华人民共和国药典》(2020年版)中何首乌项下方法,测定游离蒽醌和结合蒽醌的含量。结果大黄素8-O-β-D-葡萄糖苷、大黄素和大黄素甲醚分别在0.6745~21.5845、0.4267~13.6550和0.2003~6.9685μg·mL^(-1)范围内具有良好线性关系(R^(2)≥0.9991),平均回收率分别为98.42%、100.08%和96.60%。不同产区何首乌大黄素8-O-β-D-葡萄糖苷、大黄素、大黄素甲醚含量范围分别为0.13~2.62、0.08~0.13和0.05~0.72 mg·g^(-1);游离蒽醌和结合蒽醌含量范围分别为0.1~1.7和1.0~8.7 mg·g^(-1)。结论本方法操作简单、准确度高、重复性好,可以用于何首乌中蒽醌类成分含量测定。Objective To simultaneously determine the contents of emodin-8-O-β-D glucoside,emodin,physcion,free anthraquinone and bound anthraquinone in Polygoni Multiflori Radix from different origins and processed with different methods.Methods The contents of emodin-8-O-β-D glucoside,emodin and physcion were determined on an Agilent 5 TC-C_(18)(2)(250 mm×4.6 mm,5μm)column with a mobile phase composed of methanol and 0.1% formic acid solution with gradient elution at 1.0 mL·min^(-1).The detection wavelength was 254 nm,the column temperature 30℃,and the injection volume was 10μL.The contents of free anthraquinone and bound anthraquinone were determined using the method for Polygonum multiflorum specified in Chinese Pharmacopoeia(2020 Edition).Results Emodin-8-O-β-D glucoside,emodin,physcion had good linear relationships in the ranges of 0.6745~21.5845,0.4267~13.6550 and 0.2003-6.9685μg·mL^(-1),respectively(R^(2)≥0.9991).The recovery was 98.42%,100.08% and 96.60% respectively.The contents of emodin-8-O-β-D glucoside,emodin,physcion,free anthraquinone and bound anthraquinone in Polygoni Multiflori Radix from different origins were 0.13~2.62,0.08~0.13,0.05~0.72,0.1~1.7 and 1.0~8.7 mg·g^(-1),respectively.Conclusion This method is simple,accurate and reproducible,which can be used for the determination of anthraquinones in Polygonum multiflorum.

关 键 词：何首乌 高效液相色谱法 蒽醌类成分 含量测定 产地 炮制工艺 

分 类 号：R917[医药卫生—药物分析学]