番茄4CL基因家族鉴定和氮素处理下的表达分析  

Identification of Tomato 4CL Gene Family and Expression Analysis Under Nitrogen Treatment

作　　者：马馨馨 许洋 赵欢欢 火兆燕 王树彬[1] 钟凤林[1] MA Xin-xin;XU Yang;ZHAO Huan-huan;HUO Zhao-yan;WANG Shu-bin;ZHONG Feng-lin(College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou 350002)

机构地区：[1]福建农林大学园艺学院,福州350002

出　　处：《生物技术通报》2022年第4期163-173,共11页Biotechnology Bulletin

基　　金：设施茄果类蔬菜育种攻关与产业化工程子课题(闽财指[2021]426号);福建省科技重大专项(2018NZ0002-2);现代蔬菜产业技术体系岗位专家工作站-设施蔬菜岗位(闽财指[2019]897号)。

摘　　要：探究番茄4CL基因家族的基本理化性质、分子进化特性和其在不同浓度氮素下的表达模式。对番茄4CL基因家族成员进行全基因组鉴定,采用生物信息学方法对其进行分析,并在不同浓度氮素处理下进行qPCR验证。在番茄中共鉴定出6个4CL基因家族成员,全部成员均为稳定蛋白,有5个成员呈酸性,编码的氨基酸数在538-957之间,二级结构以无规则卷曲为主,三级结构除Sl4CL04以5bsw.1.A为模型,其他成员均以5bsr.1.A为模型。番茄4CL基因家族内含子数量在5-11之间,12个Motif在Sl4CL中排序及位置都非常保守,具有较高的同源性。启动子顺式作用元件分析发现MYB作用元件的分布最多,在不同浓度氮素处理下,6个基因表达量均有显著差异。对Sl4CL03构建过表达载体,亚细胞定位结果显示位于过氧化物酶体、叶绿体和细胞质。番茄4CL家族进化具有保守性和特异性,能够在氮素处理时进行相关机理调节。The objective is to explore the basic physical and chemical properties,molecular evolution characteristics of tomato 4CL gene family and its expression pattern under different concentrations of nitrogen.The whole genome of tomato 4CL gene family members were identified,analyzed by bioinformatics methods,and verified by qPCR under different concentrations of nitrogen treatment.As results,a total of 64CL gene family members were identified in tomato(Solanum lycopersicum),all members were stable proteins,5 members belonged to acidic protein and the number of amino acids encoded by 4CL genes in tomato ranged from 538 aa to 957 aa.The secondary structure was mainly random coils,and the tertiary structure except for Sl4CL04 used 5bsw.1.A as the model,the other members all used 5bsr.1.A as the model.The number of introns in the tomato 4CL gene family was 5-11.The 12 Motifs were very conserved in order and position in Sl4CL,and had high homology.Cis-acting elements analysis indicated that MYB binding sites were the most widely distributed in the promoter region.The expression levels of the 6 genes were significantly different under different concentrations of nitrogen.The overexpression vector was constructed for Sl4CL03,and the subcellular localization results showed that it was located in peroxisome,chloroplast and cytoplasm.In conclusion,the evolution of the tomato 4CL family is conservative and specific,and can be regulated by related mechanisms during nitrogen treatment.

关 键 词：番茄 4CL基因家族 成员鉴定 氮素 表达分析 

分 类 号：S641.2[农业科学—蔬菜学] Q943.2[农业科学—园艺学]