OX40/OX40L共刺激信号分子在慢性乙肝病毒感染者外周血中的表达研究  

Expression of OX40/OX40L Costimulatory Signaling Molecule in Peripheral Blood of Chronic Hepatitis B Virus Infected Patients.

作　　者：窦芊 李赢 王园园[1] 杨瑞[1] DOU Qian;LI Ying;WANG Yuanyuan(Department of Infection,the Third Affiliated Hospital of Xinxiang Medical University,Xinxiang City,He’nan Province 453000)

机构地区：[1]新乡医学院第三附属医院感染科,河南省新乡市453000

出　　处：《医学理论与实践》2022年第11期1813-1816,共4页The Journal of Medical Theory and Practice

基　　金：国家科技重大专项课题(2013ZX10002005)。

摘　　要：目的:探讨OX40/OX40L共刺激信号分子在慢性乙肝病毒(HBV)感染者外周血中的表达情况。方法:选取2019年6月—2020年6月本院收治的慢性HBV感染患者82例为观察组,同期选取健康体检者80例为对照组。观察组分为免疫耐受组、免疫清除组、低复制组、再活动组4个亚组,比较各亚组白蛋白/球蛋白(A/G)、总胆红素(TBIL)、谷丙转氨酶(ALT)等肝功能指标,以及肝功能与OX40/OX40L表达水平的相关性。结果:观察组外周血T细胞亚群CD_(4)^(+)/CD_(8)^(+)、CD_(4)^(+)、CD_(3)^(+)较对照组低,CD_(8)^(+)较对照组高(P<0.05)。观察组外周血CD_(4)^(+)、CD_(14)^(+)、CD_(19)^(+)T细胞表面的OX40/OX40L较对照组表达水平高(P<0.05)。4个亚组外周血CD_(4)^(+)T细胞表面的OX40表达水平比较,再活动组最高,其次是免疫清除组、低复制组,免疫耐受组最低(P<0.05);4个亚组CD _(14)^(+)、CD_(19)^(+)T细胞表面的OX40L表达比较,差异无统计学意义(P>0.05)。4个亚组肝功能指标A/G水平依次降低,ALT、TBIL水平依次升高,差异有统计学意义(P<0.05)。OX40/OX40L表达水平与肝功能指标ALT、A/G呈正相关(P<0.05),与TBIL不具相关性(P>0.05)。结论:慢性乙肝患者外周血OX40/OX40L分子表达水平与病例发展阶段、肝功能指标具有相关性,活动性越高,表达水平越高,肝功能受损越严重,借助于检测OX40/OX40L表达,可反映患者免疫功能。Objective:To investigate the expression of OX40/OX40L costimulatory signaling molecules in peripheral blood of chronic hepatitis B virus(HBV)infected patients.Methods:A total of 82 patients with chronic HBV infection admitted to our hospital from June 2019 to June 2020 were selected as the observation group,and 80 healthy subjects were selected as the control group during the same period.Observation group was divided into 4 subgroups:immune tolerance group,immune clearance group,low replication group and reactivity group.Liver function indexes such as albumin/globulin(A/G),total bilirubin(TBIL)and alanine aminotransferase(ALT)in each subgroup were compared,as well as the correlation between liver function and the expression level of OX40/OX40L.Results:The CD_(4)^(+)/CD_(8)^(+),CD_(4)^(+),CD_(3)^(+) subsets of peripheral blood T cells in the observation group were lower than those in the control group,and the CD_(8)^(+) subsets were higher than those in the control group(P<0.05).The expression level of OX40/OX40L on the surface of CD_(4)^(+),CD_(14)^(+) and CD_(19)^(+)T cells in peripheral blood of observation group was higher than that of control group(P<0.05).The expression level of OX40 on the surface of CD_(4)^(+)T cells in peripheral blood of the 4 subgroups was the highest in the reactivity group,followed by the immune clearance group,the low replication group,and the immune tolerance group(P<0.05).There was no significant difference in the expression of OX40L on the surface of CD_(14)^(+) and CD_(19)^(+)T cells among 4 subgroups(P>0.05).The level of liver function index A/G in 4 subgroups decreased successively,while the levels of ALT and TBIL increased successively,with statistical significance(P<0.05).The expression level of OX40/OX40L was positively correlated with liver function indexes ALT and A/G(P<0.05),but had no correlation with TBIL(P>0.05).Conclusion:The expression level of OX40/OX40L molecule in peripheral blood of patients with chronic hepatitis B is correlated with the development stage of the

关 键 词：慢性乙肝 OX40/OX40L T细胞 共刺激分子 

分 类 号：R512.62[医药卫生—内科学]