丹参酮ⅡA改善心肌梗死大鼠心脏功能、心肌细胞自噬及Notch通路的实验研究  

Experimental study of Tanshinone ⅡA on improvement of cardiac function, myocardial autophagy and Notch pathway in rats with myocardial infarction

作　　者：武爽 王小龙 徐晶[1] 王宏兰[1] 郭俊杰[1] 赵正林 李林[1] WU Shuang;WANG Xiao-long;XU Jing(Department of Biochemistry,Qiqihar Medical College,Qiqihar 161000,China)

机构地区：[1]齐齐哈尔医学院生物化学教研室,黑龙江齐齐哈尔161000

出　　处：《中国处方药》2022年第5期18-20,共3页Journal of China Prescription Drug

基　　金：黑龙江省中医药科研项目(ZHY18-165)。

摘　　要：目的研究丹参酮ⅡA(TSⅡA)对心肌梗死(MI)大鼠心脏功能、心肌细胞自噬及Notch通路的调节作用。方法选择成年雄性SD大鼠并分为对照组、MI组、TSⅡA组,后两组采用左冠状动脉前降支结扎的方式建立MI模型,TSⅡA组给予50 mg/kg TSⅡA腹腔注射、连续3周。比较三组大鼠心脏功能超声指标、血清心肌损伤标志物、心肌中凋亡基因及Notch通路基因表达的差异。结果MI组大鼠的LVEF、LVFS水平及心肌中P62、Notch1、Hes1、HIF-1α的表达水平均明显低于对照组,血清中CK、CK-MB、LDH的含量及心肌中LC3-II/LC3-I、Beclin-1、PTEN的表达水平明显高于对照组;TSⅡA组大鼠的LVEF、LVFS水平及心肌中P62、Notch1、Hes1、HIF-1α的表达水平均明显高于MI组,血清中CK、CK-MB、LDH的含量及心肌中LC3-II/LC3-I、Beclin-1、PTEN的表达水平明显低于MI组。结论丹参酮ⅡA对MI大鼠的心脏功能、心肌细胞自噬具有改善作用,且该作用可能与Notch通路的激活有关。Objective To study the regulatory effects of tanshinone ⅡA(TSⅡA)on cardiac function,autophagy of myocardial cells and Notch pathway in rats with myocardial infarction(MI).Methods Adult male SD rats were divided into control group,MI group and TSⅡA group.MI model was established by ligation of left anterior descending coronary artery in the latter two groups.TSⅡA group was given intraperitoneal injection of 50 mg/kg TSⅡA for 3 weeks.The differences of cardiac function ultrasound parameters,serum myocardial injury markers,apoptotic genes and Notch pathway gene expression were compared among the three groups.Results Levels of LVEF,LVFS and expressions of P62,Notch1,Hes1 and HIF-1a in myocardium of rats in MI group were significantly lower than those in control group,the levels of CK,CK-MB,LDH in serum and expressions of LC3-Ⅱ/LC3-I,Beclin-1 and PTEN in myocardium of rats were significantly higher than those in control group.Levels of LVEF,LVFS and expressions of P62,Notch1,Hes1 and HIF-1a in myocardium of rats in TSⅡA group were significantly higher than those in MI group,the levels of CK,CK-MB,LDH in serum and expressions of LC3-Ⅱ/LC3-Ⅰ,Beclin-1 and PTEN in myocardium of rats were significantly lower than those in MI group.Conclusion Tanshinone ⅡA can improve the cardiac function and autophagy of myocardial cells in MI rats,and this effect may be related to the activation of Notch pathway.

关 键 词：心肌梗死 丹参酮ⅡA 心脏功能 自噬 NOTCH通路 

分 类 号：R285.5[医药卫生—中药学]