Cytological and transcriptional analysis reveal phosphatidylinositol signaling pathway plays key role in mitotic division of Pyropia yezoensis  

作　　者：Yunke ZHU Xinran WANG Bin SUN Xianghai TANG Yunxiang MAO 

机构地区：[1]Key Laboratory of Marine Genetics and Breeding(Ministry of Education),College of Marine Life Sciences,Ocean University of China,Qingdao 266003,China [2]MOE Key Laboratory of Utilization and Conservation for Tropical Marine Bioresources,College of Fisheries and Life Science,Hainan Tropical Ocean University,Sanya 572022,China [3]Laboratory for Marine Biology and Biotechnology,Pilot National Laboratory for Marine Science and Technology(Qingdao),Qingdao 266237,China

出　　处：《Journal of Oceanology and Limnology》2022年第3期1148-1159,共12页海洋湖沼学报（英文）

基　　金：Supported by the National Key R&D Program of China(Nos.2018YFD0900106,2020YFD0901101);the Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology(Qingdao)(No.2018SDKJ0302-4);the Fundamental Research Funds for the Central Universities(No.202064006);the MOA Modern Agricultural Talents Support Project;Special Project of Central Government Guiding Local Science and Technology Development(No.7033204020)。

摘　　要：The phosphatidylinositol(PI)signaling system,a central regulator of eukaryotic metabolism,is widely found in eukaryotes for regulating a variety of cell activities.Most of the genes in the PI signaling system were found conserved in Pyropia yezoensis.In this experiment,wortmannin was used as an inhibitor to inhibit the activity of phosphatidylinositol-3 kinase(PI3K),an important regulator of the PI signaling system.After wortmannin treatment,the mitotic division of P.yezoensis was signifi cantly inhibited in a dose-dependent manner,and the mitotic division percentage was reduced by 68.1%and 91.9%in the 5-and 10-μmol/L groups,respectively.When thalli were treated with wortmannin,the levels of reactive oxygen species(ROS)were signifi cantly decreased.Furthermore,the expression level of PI3K was inhibited and the expression levels of downstream genes regulated by PI3K was signifi cantly changed.In the PI3K-AGC signaling pathway,the expression levels of Serine/threonine protein kinase(AGC)and cyclindependent kinases A(CDKA)were downregulated,while WEE1 kinase gene(WEE1)was upregulated.Three nicotinamide adenine dinucleotide phosphate(NADPH)oxidase genes were downregulated after wortmannin treatment.These results indicate that the PI signaling system plays an important role in the regulation of cell activity in P.yezoensis.It was speculated that the growth and development of P.yezoensis might be regulated by P.yezoensis PI3K,which promoted the expression of the AGC gene and further regulates the expression of downstream WEE1 and CDKA genes to advance mitotic division,and also promoted the expression level of NADPH oxidase that regulates ROS homeostasis.

关 键 词：Pyropia yezoensis phosphatidylinositol signaling system reactive oxygen species mitotic division 

分 类 号：S917.3[农业科学—水产科学]