Immunostimulatory eff ect of quaternary degree and acetyl group of quaternized chitosan on macrophages RAW 264.7  

作　　者：Xiaomin LI Rong’e XING Chaojie XU Song LIU Yukun QIN Kecheng LI Huahua YU Pengcheng LI 

机构地区：[1]CAS and Shandong Province Key Laboratory of Experimental Marine Biology,Center for Ocean Mega-Science,Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266237,China [2]Laboratory for Marine Drugs and Bioproducts,Pilot National Laboratory for Marine Science and Technology(Qingdao),Qingdao 266237,China [3]University of Chinese Academy of Sciences,Beijing 100049,China

出　　处：《Journal of Oceanology and Limnology》2022年第3期1160-1170,共11页海洋湖沼学报（英文）

基　　金：*Supported by Key Deployment Projects of the Marine Science Research Center of Chinese Academy of Sciences(No.COMS2020J04)。

摘　　要：Hydroxypropyltrimethyl ammonium chloride chitosan(HACC)and hydroxypropyltrimethyl ammonium chloride fully deacetylated chitosan(De-HACC)were synthesized with various degrees of substitution by altering the ratio of chitosan to glycidyl trimethyl-ammonium chloride(GTMAC).The effects of the quaternary ammonium degree and the acetyl group of these polymers on immunostimulatory activities were detected in RAW 264.7 cells.The expression levels of nitrogen oxide(NO),interleukin-6(IL-6)and tumor necrosis factor(TNF-α)were compared.Results show that the removal of acetyl groups in chitosan obviously improved the degree of substitution of quaternary ammonium salts.In addition,HACC and De-HACC were capable of promoting immunological activity in a substitution-dependent manner;HACC was positively correlated,and De-HACC was negatively correlated.Among tested ratios,HACC-30%and De-HACC-54%performed better than the others,and De-HACC-54%performed the best.Generally,quaternized chitosan possesses immunostimulatory activity,which is related to the degree of quaternization and the acetyl group.

关 键 词：quaternized chitosan fully deacetylated quaternized chitosan degree of quaternization Immunostimulatory activity 

分 类 号：R392[医药卫生—免疫学]