膝骨关节炎关节液和滑膜中微小RNA-140-5p的表达及临床意义  

Expression and clinical significance of miR-140-5p in synovial fluid and synovium of knee osteoarthritis

作　　者：张海英[1] 郑晨颖[2] ZHANG Haiying;ZHENG Chenying(Department of Orthopedics,Dongfang Hospital,Beijing University of Traditional Chinese Medicine,Beijing 100078,China;Department of Orthopedics,Dongzhimen Hospital,Beijing University of Traditional Chinese Medicine,Beijing 100700,China)

机构地区：[1]北京中医药大学东方医院骨科,北京100078 [2]北京中医药大学东直门医院骨科,北京100700

出　　处：《安徽医药》2022年第6期1179-1182,共4页Anhui Medical and Pharmaceutical Journal

摘　　要：目的研究膝骨关节炎(KOA)病人关节液和滑膜中微小RNA(miR)-140-5p的表达及临床意义。方法选取2018年5月至2019年5月于北京中医药大学东方医院就诊的51例KOA病人为观察组,依据凯尔格伦-劳伦斯(K-L)分级标准将观察组分为3个亚组:KOAⅡ组(12例)、KOAⅢ组(24例)、KOAⅣ组(15例),另外选取55例同期该院因骨折、截肢等手术治疗的无骨关节炎病人为对照组。采用实时荧光定量逆转录聚合酶链反应(qRT-PCR)检测并比较各组关节液和滑膜中miR-140-5p的表达,分析miR-140-5p与KOA病情进展的关系;采用受试者工作曲线(ROC)分析miR-140-5p对KOA的诊断价值。结果观察组关节液(0.51±0.16)、滑膜(0.67±0.21)中miR-140-5p均比对照组[(1.08±0.38)、(1.54±0.35)]低(P<0.05);KOAⅡ组、KOAⅢ组、KOAⅣ组相比,关节液[(0.65±0.17)、(0.51±0.14)、(0.42±0.12)]、滑膜[(0.86±0.22)、(0.69±0.15)、(0.49±0.13)]中miR140-5p均呈依次降低趋势,两两相比差异有统计学意义(P<0.05);Spearman相关性分析结果显示,KOA病人关节液、滑膜中miR-140-5p与K-L分级均呈负相关(P<0.05);ROC结果显示,关节液、滑膜miR-140-5p诊断KOA的曲线下面积(AUC)分别为0.913、0.931,截断值分别是0.78、1.05,灵敏度、特异度、准确度、约登指数、阳性预测值、阴性预测值、阳性似然比、阴性似然比分别为98.04%、78.18%、87.74%、0.76、80.65%、97.73%、4.49、0.03;96.08%、89.09%、92.45%、0.85、89.09%、96.08%、8.81、0.04。结论miR-140-5p在KOA病人关节液及滑膜中均呈低表达,与K-L分级均呈负相关,可能成为KOA病人病情发展的监测指标及治疗靶点。Objective To study the expression and clinical significance of microRNA-140-5p(miR-140-5p)in synovial fluid and synovium of patients with knee osteoarthritis(KOA).Methods A total of 51 patients with KOA treated in Dongfang Hospital of Beijing University of Traditional Chinese Medicine from May 2018 to may 2019 were selected as the observation group.According to the Kelgren Lawrence(K-L)classification standard,the observation group was divided into three subgroups:KOAⅡgroup(12 cases),KOAⅢgroup(24 cases),KOAⅣgroup(15 cases).In addition,55 patients without osteoarthritis treated by fracture and amputation in the hospital in the same period were selected as the control group.The expression of miR-140-5p in synovial fluid and synovium of each group was detected by real-time fluorescence quantitative reverse transcription polymerase chain reaction(qRT-PCR),the relationship between miR-140-5p and the progression of KOA was analyzed;and the diagnostic value of miR-140-5p to KOA was analyzed by receiver operating characteristic curve(ROC).Results MiR-140-5p in synovial fluid(0.51±0.16)and synovium(0.67±0.21)of the KOA group was lower than that of the control group[(1.08±0.38),(1.54±0.35)](P<0.05);miR-140-5p in synovial fluid[(0.65±0.17),(0.51±0.14),(0.42±0.12)]and synovium[(0.86±0.22),(0.69±0.15),(0.49±0.13)]in KOAⅡgroup,KOAⅢgroup and KOAⅣgroup decreased in turn,the differences between any two groups were statistically significant(P<0.05);Spearman's results showed that miR-140-5p in synovial fluid and synovium of KOA patients were negatively correlated with K-L grading(P<0.05);the results of ROC showed that the areas under the curve(AUC)for diagnosing KOA were 0.913 and 0.931(P<0.05),respectively,the cutoff values were 0.78 and 1.05,respectively,and the sensitivity,specificity,accuracy,Youden index,positive predictive value,negative predictive value,positive likelihood ratio and negative likelihood ratio were 98.04%,78.18%,87.74%,0.76,80.65%,97.73%,4.49,0.03;96.08%,89.09%,92.45%,0.85,89.09%,96.08%,8.

关 键 词：骨关节炎 膝 关节液 滑膜 微小RNA-140-5p 

分 类 号：R684.3[医药卫生—骨科学]