甲状腺肿瘤患者生化免疫检验中化学发光免疫测定技术的应用分析  

Application of chemiluminescence immunoassay in biochemical immunoassay of thyroid tumor patients

作　　者：郝振伟 HAO Zhenwei(Department of Clinical Laboratory,Jiamusi Hospital of Traditional Chinese Medicine in Heilongjiang Province,Jiamusi 154002,China)

机构地区：[1]黑龙江省佳木斯市中医医院检验科,黑龙江佳木斯154002

出　　处：《中国现代医生》2022年第15期126-128,158,共4页China Modern Doctor

摘　　要：目的 探讨甲状腺肿瘤患者生化免疫检验中化学发光免疫测定技术的应用价值。方法 选取2019年5月至2021年5月佳木斯市中医医院90例疑似甲状腺肿瘤患者作为研究对象,所有患者均接受生化免疫检验,检验方法分别为化学发光免疫测定法(CLIA)与免疫放射分析法(RIA),对比两种方法对甲状腺激素及甲状腺球蛋白水平、个数检测差异性,将病理诊断作为金标准,分析两种方法对疾病检出情况及敏感度、特异度、准确性。结果CLIA测定法检验甲状腺球蛋白水平、甲状腺球蛋白个数均高于RIA组,差异有统计学意义(P<0.05);CLIA测定法检验FT3、FT4及TSH均高于RIA组,差异有统计学意义(P<0.05);病理学证实,90例患者中72例为阳性,18例为阴性,CLIA测定法显示阳性69例,阴性17例,RIA测定法显示阳性53例,阴性11例;CLIA测定法诊断甲状腺肿瘤敏感度、特异度及准确性分别为95.83%、94.44%、95.56%,高于RIA测定法的72.22%、61.11%、70.00%,差异有统计学意义(P<0.05)。结论 CLIA测定法应用于甲状腺肿瘤患者生化检验,敏感度、特异度及准确性高,能够为甲状腺肿瘤早期诊断及治疗方案选择提供指导,具有较高临床价值。Objective To explore the application value of chemiluminescence immunoassay in biochemical immunoassay of thyroid tumor patients. Methods A total of 90 patients with suspected thyroid tumor in Jiamusi Hospital of Traditional Chinese Medicine from May 2019 to May 2021 were selected as the research objects,and all patients received biochemical immunoassay. Chemiluminescence immunoassay(CLIA) and radioimmunoassay(RIA)were used respectively.The differences of thyroid hormone and thyroglobulin levels and numbers detected by the two methods were compared, and pathological diagnosis was taken as the gold standard to analyze the disease detection situation, sensitivity, specificity and accuracy of the two methods. Results The level and number of thyroglobulin detected by CLIA were higher than those of the RIA group, and the differences were statistically significant(P<0.05).Levels of FT3, FT4and TSH in the CLIA group were higher than those in the RIA group, and the differences were statistically significant(P<0.05).Pathology confirmed that 72 of the 90 patients were positive and 18 were negative;CLIA showed positive in 69 and negative in 17;RIA showed positive in 53 and negative in 11.The sensitivity,specificity and accuracy of CLIA were 95.83%, 94.44% and 95.56%, respectively,which were higher than 72.22%, 61.11% and70.00% of RIA, and the differences were statistically significant(P <0.05). Conclusion CLIA has high sensitivity,specificity and accuracy in biochemical examination of thyroid tumor patients, which can provide guidance for early diagnosis and treatment options of thyroid tumor, and has a high clinical value.

关 键 词：甲状腺肿瘤 生化免疫检验 化学发光免疫测定技术 免疫放射分析法 敏感度 

分 类 号：R446.1[医药卫生—诊断学]