酰基辅酶A合成酶中链家族成员3、微小RNA-653-5p在前列腺癌组织中的表达及其与患者临床病理特征、预后的关系  

The relationship between the expression of ACSM3 and miR-653-5p in prostate cancer tissues and the clinicopathological characteristics and prognosis of patients

作　　者：李伟 谢喜[1] 李峰[1] 王鹏桥[1] 罗鹏伟 张园园 LI Wei;XIE Xi;LI Feng;WANG Pengqiao;LUO Pengwei;ZHANG Yuanyuan(Department of Urinary Surgery,the First Afiliated Hospial of Chengdu Medical College,Chengdu 610000,Sichuan,China;Department of Medical Inspection,Xindu District People's Hospital of Chengdu City,Chengdu 610000,Sichuan,China)

机构地区：[1]成都医学院第一附属医院泌尿外科,成都610000 [2]成都市新都区人民医院医学检验科,成都610000

出　　处：《中国性科学》2022年第5期9-13,共5页Chinese Journal of Human Sexuality

基　　金：国家重点研发计划(2019YFF0216501-Z05)。

摘　　要：目的 检测酰基辅酶A合成酶中链家族成员3(ACSM3)、微小RNA-653-5p(miR-653-5p)在前列腺癌组织中的表达,并探讨两者与患者临床病理特征及预后的关系。方法 收集2016年4月至2018年4月成都医学院第一附属医院收治的150例行前列腺癌根治术患者的前列腺癌组织标本、癌旁组织标本作为研究对象。采用实时荧光定量聚合酶链反应(qRT-PCR)法检测前列腺癌组织、癌旁组织中ACSM3 mRNA、miR-653-5p的表达水平;分析前列腺癌组织中ACSM3 mRNA、miR-653-5p水平与患者临床病理特征的关系;采用Kaplan-Meier生存曲线分析前列腺癌组织中ACSM3、miR-653-5p表达水平与患者预后的关系。结果 与癌旁组织相比,前列腺癌组织中ACSM3 mRNA水平显著降低,miR-653-5p水平显著升高(P<0.05);Gleason评分≥8分、TNM分期为Ⅲ~Ⅳ期、分化程度为未-低分化、有淋巴结转移的前列腺癌患者的前列腺癌组织中ACSM3 mRNA水平均低于Gleason评分<8分、TNM分期为Ⅰ~Ⅱ期、分化程度为中-高分化、无淋巴结转移的前列腺癌患者,而miR-653-5p水平均高于Gleason评分<8分、TNM分期为Ⅰ~Ⅱ期、分化程度为中-高分化、无淋巴结转移的前列腺癌患者(P<0.05);ACSM3 mRNA低表达患者3年总体生存率(60.49%)低于ACSM3 mRNA高表达患者(85.51%)(χ^(2)=11.564,P<0.05);miR-653-5p低表达患者3年总体生存率(80.56%)高于miR-653-5p高表达患者(64.10%)(χ^(2)=5.027,P<0.05)。结论 前列腺癌组织中ACSM3表达降低,miR-653-5p表达升高,两者表达水平与患者的临床病理特征关系密切,且能预测患者术后3年的生存情况。Objective To detect the expression of Acyl-CoA synthetase medium-chain family member 3(ACSM3)and microRNA-653-5 p(miR-653-5 p)in prostate cancer tissues,and explore the relationship between the two and the patient′s clinicopathological characteristics and prognosis.Methods From April 2016 to April 2018,the specimens of prostate cancer tissues,adjacent tissues and clinicopathological features of 150 patients with radical resection of prostate cancer were collected from the First Affiliated Hospital of Chengdu Medical College.Real-time fluorescence quantitative polymerase chain reaction(qRT-PCR)method was used to detect the levels of ACSM3 mRNA and miR-653-5 p in prostate cancer tissues and adjacent tissues;the relationship between ACSM3 mRNA and miR-653-5 p levels in prostate cancer tissues and clinicopathological features of patients was analyzed;and the Kaplan-Meier survival curve was used to analyze the relationship between the expression levels of ACSM3 and miR-653-5 p in prostate cancer tissue and prognosis.Results Compared with adjacent tissues,the expression level of ACSM3 mRNA in prostate cancer tissue was significantly reduced,and the expression level of miR-653-5 p was significantly increased(P<0.05);ACSM3 mRNA levels in prostate cancer tissues of patients with Gleason score≥8 score,TNM stageⅢ-Ⅳ,undifferentiated and poorly differentiated,and lymph node metastasis were all lower than Gleason score<8 score,TNM stageⅠ-Ⅱ,medium to high differentiation and no lymph node metastasis,while the miR-653-5 p levels were higher than that of the prostate cancer patients with Gleason score<8 score,TNM stageⅠ-Ⅱ,medium to high differentiation and no lymph node metastasis(P<0.05);the 3-year overall survival rate(60.49%)of patients with low ACSM3 mRNA expression was lower than the 3-year overall survival rate(85.51%)of patients with high ACSM3 mRNA expression(χ^(2)=11.564,P<0.05);the 3-year overall survival rate(80.56%)of patients with low miR-653-5 p expression was higher than the 3-year overall survival

关 键 词：酰基辅酶A合成酶中链家族成员3 微小RNA-653-5p 前列腺癌 临床病理特征 预后 

分 类 号：R691[医药卫生—泌尿科学]