骨桥蛋白基因沉默对子宫内膜异位症患者在位内膜细胞侵袭性的影响  

Effect of osteopontin gene silencing on invasiveness of eutopic endometrial cells in patients with endometriosis

作　　者：白治苗 刘玉锋 郭玉琳[1] 赵乐 BAI Zhimiao;LIU Yufeng;GUO Yulin;ZHAO Le(Department of Gynecology,Yulin No.2 Hospital,Yulin 719053,China)

机构地区：[1]榆林市第二医院妇科,陕西榆林719053 [2]榆林市第二医院神经外科,陕西榆林719053

出　　处：《陕西医学杂志》2022年第6期668-671,共4页Shaanxi Medical Journal

基　　金：陕西省榆林市科技计划项目(YF-2020-055)。

摘　　要：目的:探讨骨桥蛋白(OPN)基因沉默对子宫内膜异位症(EMS)患者在位内膜细胞侵袭性的影响。方法:采用原代细胞培养方法培养EMS患者在位内膜腺上皮细胞,分为干预组和未干预组。干预组采用OPN特异小RNA(siRNA)进行基因沉默。采用Western blot、RT-PCR检测细胞中OPN蛋白、mRNA表达情况。采用穿膜实验观察细胞的侵袭性变化。结果:与未干预组比较,干预组细胞OPN蛋白及mRNA表达明显下降(均P<0.05)。与未干预组比较,干预组细胞穿膜数减少(P<0.05)。结论:对EMS患者在位内膜腺上皮细胞进行OPN基因沉默后,细胞OPN蛋白、mRNA表达降低,侵袭性明显减弱,说明OPN与细胞侵袭性明显相关。Objective:To investigate the effect of osteopontin(OPN)gene silencing on the invasiveness of eutopic endometrial cells in patients with endometriosis(EMS).Methods:The epithelial cells of eutopic endometrium from EMS patients were cultured by primary cell culture and divided into intervention group and non-intervention group.In the intervention group,OPN interfering small RNA(siRNA)was used for gene silencing.Western blot and RT-PCR were used to detect the expression of OPN protein and mRNA in cells.The change of cellular invasiveness was observed by transmembrane test.Results:Compared with the non-intervention group,the expressions of OPN protein and mRNA in the intervention group was significantly decreased(all P<0.05).Compared with the non-intervention group,the number of cells penetrating the membrane decreased in the intervention group(P<0.05).Conclusion:After OPN gene silencing of EMS glandular epithelial cells,the expressions of OPN protein and mRNA decreased,and the invasiveness decreased,which indicated that OPN was significantly related to cell invasiveness.

关 键 词：子宫内膜异位症 骨桥蛋白 腺上皮细胞 细胞侵袭性 基因沉默 

分 类 号：R711.71[医药卫生—妇产科学]