上海市水稻田千金子对3种乙酰辅酶A羧化酶抑制剂的抗性现状及酶突变机制  

Resistance status of Leptochloa chinensis to three acetyl-CoA carboxylase(ACCase)inhibitors in rice fields in Shanghai and involved ACCase gene mutations

作　　者：袁国徽 田志慧[1] 高原 沈国辉[1] YUAN Guohui;TIAN Zhihui;GAO Yuan;SHEN Guohui(Eco-Environmental Protection Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai 201403,China)

机构地区：[1]上海市农业科学院生态环境保护研究所,上海201403

出　　处：《农药学学报》2022年第3期492-500,共9页Chinese Journal of Pesticide Science

基　　金：上海市科技兴农重点攻关项目(2020-02-08-00-12-F01490);上海市农业科学院卓越团队项目(农科创2018 B-01).

摘　　要：为明确上海市水稻田千金子对乙酰辅酶A羧化酶(ACCase)抑制剂类除草剂的抗性发生情况及可能存在的抗性机制,在上海市千金子发生严重地区的水稻田共采集51个种群,采用单剂量抗性甄别法测定了不同千金子种群对3种ACCase抑制剂类除草剂的抗性水平,扩增和比对了靶标酶ACCase基因部分片段的差异。结果显示:在氰氟草酯105 g/hm^(2)有效成分剂量选择压下,8个千金子种群标记为抗性种群,6个种群为发展中抗性种群;在噁唑酰草胺120 g/hm^(2)有效成分剂量选择压下,4个千金子种群标记为抗性种群,5个种群为发展中抗性种群;在精噁唑禾草灵62.1 g/hm^(2)有效成分剂量选择压下,6个千金子种群标记为抗性种群,2个种群为发展中抗性种群。对15个抗性千金子种群靶标酶基因片段的测序发现,9个种群共发生了4种ACCase基因突变类型,分别为ACCase基因1999位点色氨酸(TGG)突变为丝氨酸(TCG)、1999位点色氨酸(TGG)突变为半胱氨酸(TGT)、2027位点色氨酸(TGG)突变为丝氨酸(TCG)以及2027位点色氨酸(TGG)突变为半胱氨酸(TGT/TGC)。其中,5个抗性千金子种群ACCase基因突变频率均大于60%。研究表明,ACCase抑制剂类除草剂抗性千金子在上海市部分地区发生已较为严重,ACCase基因突变是导致不同千金子种群对该类除草剂产生抗性的重要原因之一。In order to determine the resistance level and the potential resistance mechanism of Leptochloa chinensis to acetyl-CoA carboxylase(ACCase)-inhibiting herbicides,51 populations of L.chinensis were collected from the rice fields in Shanghai.Whole-plant single-dose experiments were conducted to investigate the resistance level of different populations to ACCase-inhibiting herbicides,including cyhalofop-butyl at 105 g(a.i.)/hm^(2),metamifop at 120 g(a.i.)/hm^(2) and fenoxaprop-P-ethyl at 62.1 g(a.i.)/hm^(2) in the greenhouse.Meanwhile,the sequences of the target gene ACCase in resistant populations were analyzed.The results showed that of the 51 populations tested,eight populations were found to be resistant to clodinafop-propargyl,four populations resistant to metamifop,and six populations resistant to fenoxaprop-P-ethyl;six,five and two populations were found to be developing resistance to cyhalofop-butyl,metamifop and fenoxaprop-P-ethyl,respectively.Sequencing results showed that nine of fifteen resistant populations showed four different kinds of mutations in ACCase sequence,namely Trp(TGG)-1999-Ser(TCG),Trp(TGG)-1999-Cys(TGT),Trp(TGG)-2027-Ser(TCG)and Trp(TGG)-2027-Cys(TGT/TGC),but there were no mutations in other populations.Besides,five populations had mutant frequencies higher than 60%.Our results indicated that L.chinensis was extensively resistant to ACCase-inhibiting herbicides in some rice fields in Shanghai,and the mutations in the ACCase gene were one of the main causes of the resistance of L.chinensis populations to ACCase-inhibiting herbicides.

关 键 词：千金子 乙酰辅酶A羧化酶 氰氟草酯 噁唑酰草胺 精噁唑禾草灵 靶标抗性 基因突变 

分 类 号：S481.4[农业科学—农药学] S482.4[农业科学—植物保护]