两个取代苄基锡配合物的合成、抗癌活性及其与DNA相互作用  

Synthesis,Anti⁃tumor Activity,and Interaction with DNA of Two Substituted Benzyltin Complexes

作　　者：陈乐 邓欣 谭宇星 张复兴 邝代治 蒋伍玖 CHEN Le;DENG Xin;TAN Yu-Xing;ZHANG Fu-Xing;KUANG Dai-Zhi;JIANG Wu-Jiu(Key Laboratory of Functional Metal-Organic Compounds of Hunan Province,Key Laboratory of Organometallic New Materials of College of Hunan Province,Hunan Provincial Engineering Research Center for Monitoring and Treatment of Heavy Metals Pollution in the Upper Reaches of Xiangjiang River,College of Chemistry and Materials Science,Hengyang Normal University,Hengyang,Hunan 421008,China)

机构地区：[1]衡阳师范学院化学与材料科学学院,金属有机新材料湖南省高校重点实验室,功能金属有机化合物湖南省重点实验室,湘江上游重金属污染监测与治理湖南省工程研究中心,衡阳421008

出　　处：《无机化学学报》2022年第6期1081-1089,共9页Chinese Journal of Inorganic Chemistry

基　　金：衡阳师范学院大学生创新创业训练计划项目(No.cxcy2021072)和衡阳师范学院大学生课外学术科技作品竞赛项目(No.14)资助

摘　　要：利用二(2,4-二氯苄基)二氯化锡分别与对甲基苯甲酰肼或对叔丁基苯甲酰肼、丙酮酸钠在甲醇中发生反应,合成了2个二(2,4-二氯苄基)锡配合物(C1、C2),通过元素分析、IR、^(1)H NMR、^(13)C NMR、^(119)Sn NMR、HRMS以及X射线单晶衍射表征了配合物结构。测试了配合物C1、C2的热稳定性以及配合物对NCI-H460(人肺癌细胞)、HepG2(人肝癌细胞)和MCF7(人乳腺癌细胞)的体外抑制活性,发现配合物C1对癌细胞均表现较好的抑制作用。利用UV-Vis光谱、荧光光谱以及黏度法研究了2个配合物与ct-DNA之间的相互作用,结果表明配合物是以经典的嵌入模式与DNA结合。Two organotin complexes(C1,C2)were synthesized by di(2,4-dichlorobenzyl)tin dichloride reacting with p-methyl benzoyl hydrazide or p-tert-butyl benzoyl hydrazide,and sodium pyruvate.The structure of the complexes were characterized through elemental analysis,IR,^(1)H NMR,^(13)C NMR,^(119)Sn NMR,HRMS,and X-ray single crystal diffraction.The thermal stability of complexes C1 and C2 were analyzed,and the antitumor activities of the complexes were evaluated by MTT against three cell lines(human lung cancer cells NCI-H460,human liver cancer cells HepG2,and human breast cancer cells MCF7).It was found that complex C1 showed a good inhibitory effect on NCI-H460,HepG2,and MCF7.The interaction of the complexes with DNA was investigated using UV-Vis spectroscopy,fluorescence spectroscopy,and viscosity measurement.It is found that the complexes can bind to DNA through an intercalation mode.CCDC:2124763,C1;2124764,C2.

关 键 词：有机锡配合物 合成 晶体结构 抗肿瘤 脱氧核糖核酸 

分 类 号：O614.432[理学—无机化学]