谷胱甘肽和丁硫堇对镉胁迫下辣椒幼苗生理特性的影响  

Effects of GSH and BSO on physiological characteristics of pepper seedlings under cadmium stress

作　　者：雷阳 乔宁 苗如意 杨玉花 梁燕萍 LEI Yang;QIAO Ning;MIAO Ru-yi;YANG Yu-hua;LIANG Yan-ping(College of Horticulture,Shanxi Agriculture University,Taiyuan Shanxi 030031;Center for Agricultural Genetic Resources Research,Shanxi Agricultural University,Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau,Ministry of Agriculture and Rural Affairs,Taiyuan Shanxi 030031)

机构地区：[1]山西农业大学园艺学院,山西太原030031 [2]山西农业大学农业基因与资源研究中心,农业农村部黄土高原作物基因资源与种质创制重点实验室,山西太原030031

出　　处：《中国土壤与肥料》2022年第4期209-217,共9页Soil and Fertilizer Sciences in China

基　　金：山西省青年基金(20210302124152);中央引导地方科技发展专项资金项目(YDZJSX2021D003)。

摘　　要：为探究谷胱甘肽(GSH)在辣椒响应镉胁迫过程中的生理作用,在镉胁迫下测定了GSH和GSH合成抑制剂丁硫堇对辣椒叶部和根部各项生理指标的影响。结果表明,100μmol/L的镉离子对辣椒幼苗的生长有严重的抑制作用,在镉胁迫下喷施10 mmol/L的GSH可以增加辣椒幼苗的根长、株高以及地下部和地上部鲜重,与单独的镉处理相比增幅分别为22.01%、6.59%、39.11%和26.47%;叶绿素a、叶绿素b、类胡萝卜素含量分别增加了82.65%、51.33%和32.19%;根部和叶部的镉含量分别增加了11.26%和33.26%;超氧化物歧化酶、过氧化氢酶、抗坏血酸过氧化物酶、过氧化物酶、单脱氢抗坏血酸还原酶、脱氢抗坏血酸还原酶、谷胱甘肽还原酶、谷胱甘肽过氧化物酶在根部的活性分别提高49.63%、68.15%、51.60%、59.72%、56.78%、17.48%、59.53%和23.27%,在叶部分别提高14.22%、28.11%、64.86%、40.47%、28.02%、14.30%、28.60%和47.46%;GSH循环系统还原态/氧化态比例在叶部和根部分别提高了93.78%和40.32%,进而降低了超氧阴离子产生速率和H2O2含量;添加GSH合成抑制剂丁硫堇则会逆转上述作用。结果说明,GSH可有效缓解镉胁迫对辣椒造成的生理损害,进而提高辣椒抵抗重金属胁迫的能力。In order to explore the physiological role of glutathione(GSH)in pepper response to cadmium stress,the effects of GSH and GSH synthesis inhibitor buthionine sulfloximine(BSO)on various physiological indexes of pepper leaves and roots were determined under cadmium stress.The results showed that 100μmol/L cadmium ions seriously inhibited the growth of pepper seedlings.Spraying 10 mmol/L GSH under cadmium stress increased the root length,plant height,fresh weight of underground parts and aboveground parts of pepper seedlings,which increased by 22.01%,6.59%,39.11%and 26.47%,respectively,compared with Cd-only treatment;The contents of chlorophyll a,chlorophyll b and carotenoid increased by 82.65%,51.33%and 32.19%,cadmium content in roots and leaves increased by 11.26%and 33.26%respectively;The activities of superoxide dismutase,catalase,ascorbate peroxidase,peroxidase,dehydroascorbate reductase,dehydroascorbate reductase,glutathione reductase and glutathione peroxidase in roots increased by 49.63%,68.15%,51.60%,59.72%,56.78%,17.48%,59.53%and 23.27%,respectively;while in leaves increased by 14.22%,28.11%,64.86%,40.47%,28.02%,14.30%,28.60%and 47.46%,respectively;The ratio of reduced state to oxidized state in GSH circulation system increased by 93.78%and 40.32%in leaves and roots,respectively,thus reducing the production rate of superoxide anion and the content of H2O2.Adding BSO,an inhibitor of GSH synthesis,would reverse the above effects.GSH can effectively alleviate the physiological damage of pepper caused by cadmium stress,and then improve the ability of pepper to resist heavy metal stress.

关 键 词：镉胁迫 谷胱甘肽 丁硫堇 辣椒 生理特性 

分 类 号：S641.3[农业科学—蔬菜学] X173[农业科学—园艺学]