一株百合内生细菌Burkholderia sp.FJb-2的分离鉴定及其体外抑菌促生效应  

Isolation and identification of an endophytic bacterium Burkholderia sp.FJb-2 from lily and its in vitro antibacterial and growth-promoting effect

作　　者：孙雨晨 易欣欣[1] 王丽伟 程兆伦 张秀海[2] 高俊莲[2] SUN Yu-chen;YI Xin-xin;WANG Li-wei;CHENG Zhao-lun;ZHANG Xiu-hai;GAO Jun-lian(College of Food Science and Engineering,Beijing University of Agriculture,Beijing 102206;Beijing Agro-biotechnology Research Center,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097;Housing and Urban-rural Development Bureau of Mengyin County,Mengyin Shandong 276200)

机构地区：[1]北京农学院食品科学与工程学院,北京102206 [2]北京市农林科学院,北京农业生物技术研究中心,北京100097 [3]蒙阴县住房和城乡建设局,山东蒙阴276200

出　　处：《中国土壤与肥料》2022年第4期229-236,共8页Soil and Fertilizer Sciences in China

基　　金：切花和盆花轻简高效栽培技术集成与示范(2020YFD1000400);北京市农林科学院财政专项(KJCX20200426);北京市农林科学院科技创新能力建设专项(KJCX20200110)。

摘　　要：从北京市房山区百合资源圃采集红芯百合(Fangio)植株样品,通过组织块分离法分离内生细菌;进一步以百合灰霉病菌—灰葡萄孢菌(Botrytis cinerea)ACCC36423、百合叶尖干枯病菌—葡萄座腔菌(Botryosphaeria dothidea)ACCC37263和百合枯萎病菌—尖孢镰刀菌(Fusarium oxysporum)F01139等几种主要百合病原真菌为指示菌,采用平板对峙法筛选具有拮抗活性的拮抗菌株;并对拮抗菌株进行产1-氨基环丙烷-1-羧酸(ACC)脱氨酶活性、产生长素(IAA)、溶磷、产铁载体等多种植物促生特性测定;最后通过形态观察及16S rRNA基因序列分析,初步鉴定其种属。结果从百合茎部分离获得一株内生细菌菌株FJb-2;该菌株对灰葡萄孢菌、葡萄座腔菌和尖孢镰刀菌均有较高拮抗活性,对病原真菌生长的抑制率范围为60%~81%;该菌株产ACC脱氨酶活性为(35.28±0.05)μmolα-酮丁酸/(mg·h),同时具有产IAA、溶磷、产铁载体等多种植物促生特性;通过16S rRNA基因序列分析,初步鉴定FJb-2菌株为Burkholderia属细菌。结论认为百合内生细菌Burkholderia sp.FJb-2于体外条件下对多种百合病原真菌具有高效拮抗作用,并具有促进植物生长的潜力。Samples of lily plants-Fangio were collected from the Lily Resource Nursery in Fangshan District,Beijing,and some endophytic bacteria were isolated by the method of tissue block separation;Antagonistic strains with antagonistic activity against the strains of several major lily pathogens were screened by the plate confrontation method,using lily gray mold-Botrytis cinerea ACCC36423,lily leaf wilt-Botryosphaeria dothidea ACCC37263 and Fusarium oxysporum F01139and other major lily pathogenic fungi as the indicator bacteria.The antagonistic strains were tested for the production of 1-aminocyclopropane-1-carboxylic acid(ACC)deaminase,production of indole acetic acid(IAA),phosphate solubilization,production of siderophore,and then the strain was identified preliminarily according to its morphological observation and the sequence analysis of 16S rRNA gene.An endophytic bacterial strain named FJb-2 was isolated from the stem of lily;the strain FJb-2 showed the high antagonistic activity against Botrytis cinerea,Botryosphaeria dothidea and Fusarium oxysporum,and the growth inhibition rate of pathogenic fungi ranged from 60%to 81%.This strain also had ACC deaminase activity of(35.28±0.05)μmolα-ketobutyric acid/(mg·h)and had some plant growth-promoting characteristics such as IAA production,phosphorus solubilization and siderophore production.The FJb-2 strain was preliminarily identified as a Burkholderia bacterium based on the 16S rRNA gene sequence analysis.It is concluded that the lily endophytic bacterium Burkholderia sp.FJb-2 has a highly effective antagonistic effect against a variety of lily pathogenic fungi under in vitro conditions,and has the potential to promote plant growth.

关 键 词：百合 内生细菌 伯克霍尔德氏菌 抗病性 促生长特性 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]