过表达海藻糖合成酶编码基因TvTPS/TPP的绿色木霉工程菌的构建及其抗逆特性分析  

Construction and Stress Resistance Analysis of Trichoderma viride Engineering Strain Overexpressing TvTPS/TPP Gene Encoding Trehalose Bifunctional Synthase

作　　者：张豪 李哲 郭凯 郝永任 Zhang Hao;Li Zhe;Guo Kai;Hao Yongren(Biology Institute,Qilu University of Technology(Shandong Academy of Sciences),Jinan 250014,China)

机构地区：[1]齐鲁工业大学(山东省科学院)生物研究所,山东济南250014

出　　处：《山东农业科学》2022年第5期1-9,共9页Shandong Agricultural Sciences

基　　金：山东省自然科学基金项目(ZR202102270296);国家自然科学基金项目(21607169,3227010260);国家重点研发计划项目(2017YFD0200806);山东省科学院青年科学基金项目(2020QN0019,2020QN0017);济南市创新团队项目(2019GXRC033)。

摘　　要：绿色木霉广泛用于促生抗逆、生物防治、纤维素酶生产和生物质利用等方面。木霉体内代谢途径丰富,代谢产物繁多,很多代谢产物参与调控木霉对不利环境的适应能力。海藻糖是一种典型的应激代谢产物,在逆境条件下,可以保护生物免受伤害。海藻糖合成酶主要包括海藻糖磷酸合成酶和海藻糖磷酸磷酸酯酶。本试验从绿色木霉Tv-1511中鉴定出一个海藻糖合成双功能酶编码基因TvTPS/TPP,构建了绿色木霉TvTPS/TPP基因过表达菌株Tv-1511-TPS/TPP-OE,并对其在绿色木霉Tv-1511抗逆性中的功能进行了分析。结果表明,与野生型相比,TvTPS/TPP过表达菌株具有更高的海藻糖含量,过表达TvTPS/TPP基因显著提高了绿色木霉对盐、高温和干旱的耐受性。因此,TvTPS/TPP基因在绿色木霉生长抗逆方面可能起着重要作用。Trichoderma viride has been widely used in plant growth promotion,stress resistance,biological control,cellulase production and biomass utilization.Trichoderma is rich in metabolic pathways and metabolites,and many metabolites are involved in regulating the adaption of Trichoderma to adverse environments.Trehalose is a typical stress metabolite,which can protect organisms from damage under adverse conditions.Trehalose synthases mainly include trehalose-6-phosphate synthase(TPS)and trehalose-6-phosphate phosphatase(TPP).In this experiment,we identified a gene TvTPS/TPP from T.viride Tv-1511 which encoding a bifunctional trehalose synthase enzyme,constructed the T.viride overexpressing strain Tv-1511-TPS/TPP-OE,and analyzed its functions in stress resistance of T.viride Tv-1511.The results showed that,compared with wildtype,the TvTPS/TPP overexpression strain had higher trehalose yield,and the overexpression of TvTPS/TPP gene significantly improved the stress resistance of T.viride to salt,high temperature and drought.Therefore,TvTPS/TPP gene might play an important role in the growth and stress resistance of T.viride.

关 键 词：绿色木霉 海藻糖磷酸合成酶 海藻糖磷酸磷酸酯酶 抗逆特性 

分 类 号：S182[农业科学—农业基础科学] Q78[生物学—分子生物学]