人参与蛤蚧配方颗粒配伍对肾阳虚模型大鼠神经内分泌免疫网络的调节机制研究  

Study on regulatory mechanism of compatibility of ginseng and gecko dispensing granule on neuroendocrine immune network in model rats with kidney yang deficiency

作　　者：周蓓 朱巧凤 陈誉丹 冯莉婷 刘舒凌 吴燕春 ZHOU Bei;ZHU Qiaofeng;CHEN Yudan;FENG Liting;LIU Shuling;WU Yanchun(College of Pharmacy,Guangxi University of Chinese Medicine,Nanning 530200,China;Dept.of Pharmacy,Guangxi Agricultural Vocational College,Nanning 530007,China;Dept.of Pharmacy,Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine,Nanning 530012,China)

机构地区：[1]广西中医药大学药学院,南宁530200 [2]广西农业职业技术学院药学系,南宁530007 [3]广西中医药大学附属瑞康医院药学部,南宁530012

出　　处：《中国药房》2022年第11期1313-1319,共7页China Pharmacy

基　　金：广西自然科学基金资助项目(No.2019GXNSFAA245092);广西中医药大学2019-2021年广西一流学科建设开放课题(No.2019XK074);广西中医药大学引进博士科研启动基金项目(No.2018BS022)。

摘　　要：目的探讨人参与蛤蚧配方颗粒配伍对肾阳虚模型大鼠的调节作用机制。方法将SD雄性大鼠随机分为正常组(不造模、不给药)、模型组(造模、不给药)、金匮肾气丸组(造模,给药剂量为2.33 g/kg)、人参组(造模,给药剂量为0.53 g/kg)、蛤蚧组(造模,给药剂量为0.21 g/kg)、配伍组(造模,人参0.53 g/kg+蛤蚧0.21 g/kg)。分别测定大鼠不同时点的体质量、肛温;检测血清环磷酸腺苷(cAMP)、环磷酸鸟苷(cGMP)、促肾上腺皮质激素释放激素(CRH)、促肾上腺皮质激素(ACTH)、皮质醇(CORT)、睾酮(T)、三碘甲状腺原氨酸(T_(3))、甲状腺素(T_(4))、雌二醇(E_(2))、IgG、IgM水平;观察肾上腺、甲状腺、睾丸组织病理形态学变化;检测下丘脑CRH、促甲状腺激素释放激素(TRH)、促性腺激素释放激素(GnRh)mRNA表达水平。结果与模型组比较,配伍组大鼠肛温、血清cAMP、CRH、ACTH、CORT、T_(3)、T水平和cAMP/cGMP、T/E_(2)及下丘脑TRH、GnRH mRNA表达水平均显著升高(P<0.05或P<0.01);血清cGMP、E_(2)、IgG水平和下丘脑CRH mRNA表达水平均显著降低(P<0.05或P<0.01);肾上腺、甲状腺、睾丸组织的病理性损伤均有所改善。与单用人参或蛤蚧配方颗粒对比,配伍组大鼠肛温、T/E_(2)显著升高,下丘脑CRH mRNA表达水平显著降低(P<0.05或P<0.01)。结论人参与蛤蚧配方颗粒配伍应用对肾阳虚模型大鼠具有协同调节作用,其机制可能与下丘脑-垂体-肾上腺轴、下丘脑-垂体-甲状腺轴、下丘脑-垂体-性腺轴及免疫功能形成的神经内分泌免疫网络有关,且配伍用药优于单方用药。OBJECTIVE To explore the regulatory mechanism of compatibility of ginseng and gecko dispensing granule on kidney yang deficiency model rats.METHODS Male SD rats were randomly divided into normal group(no modeling,no administration),model group(modeling,no administration),Jinkui shenqi pill group(modeling,dose of 2.33 g/kg),ginseng group(modeling,dose of 0.53 g/kg),gecko group(modeling,dose of 0.21 g/kg)and compatibility group(modeling,ginseng 0.53 g/kg and gecko 0.21 g/kg).The body mass and anal temperature of rats were measured at different time points;the serum levels of cAMP,cGMP,CRH,ACTH,CORT,T,T_(3),T_(4),E_(2),IgG and IgM were measured;the pathomorphological changes of adrenal gland,thyroid gland and testis were observed;mRNA expression of CRH,thyroid stimulating hormone releasing hormone(TRH)and gonadotropin releasing hormone(GnRH)in hypothalamus were detected.RESULTS Compared with model group,the anal temperature,the levels of cAMP,CRH,ACTH,CORT,T_(3),T and cAMP/cGMP,T/E_(2)in serum and mRNA expressions of TRH and GnRH in hypothalamus were significantly increased in the compatibility group(P<0.05 or P<0.01);the levels of cGMP,E_(2)and IgG in serum and mRNA expression of CRH in hypothalamus decreased significantly(P<0.05 or P<0.01);the pathological injuries of adrenal gland,thyroid gland and testis were all improved.Compared with ginseng or gecko dispensing granules alone,the anal temperature and T/E_(2)of rats in the compatibility group increased significantly,and mRNA expression of CRH in hypothalamus decreased significantly(P<0.05 or P<0.01).CONCLUSIONS The compatibility of ginseng and gecko dispensing granule has a synergistic regulatory effect on kidney yang deficiency model rats,the mechanism of which may be associated with hypothalamus-pituitary-adrenal axis,hypothalamus-pituitary-thyroid axis,hypothalamus-pituitary-gonad axis and neuroendocrine immune network formed by immune function.Compatible drugs are better than single drugs.

关 键 词：人参 蛤蚧 配伍 肾阳虚 下丘脑-垂体-肾上腺轴 下丘脑-垂体-甲状腺轴 下丘脑-垂体-性腺轴 神经内分泌免疫网络 

分 类 号：R965[医药卫生—药理学]