蒙药三子散醇提物化学成分的HPLC-Q-Exactive-MS快速分析与鉴定  

Rapid analysis and identification of chemical components of ethanol extract from Mongolian medicine Sanzi san by HPLC-Q-Exactive-MS

作　　者：李君[1,2,3] 胡玉霞 张梦迪[3] 王跃武 张谦 胡格吉胡[4] 高峰 常福厚[3] LI Jun;HU Yuxia;ZHANG Mengdi;WANG Yuewu;ZHANG Qian;HU Gejihu;GAO Feng;CHANG Fuhou(The Center for New Drug Safety Evaluation and Research,Inner Mongolia Medical University,Hohhot 010110,China;Engineering Research Center of New Pharmaceutical Screening,Inner Mongolia Medical University,Hohhot 010110,China;College of Pharmacy,Inner Mongolia Medical University,Hohhot 010110,China;Dept.of Anesthesiology,the Affiliated Hospital of Inner Mongolia Medical University,Hohhot 010110,China)

机构地区：[1]内蒙古医科大学新药安全评价研究中心,呼和浩特010110 [2]内蒙古医科大学新药筛选工程研究中心,呼和浩特010110 [3]内蒙古医科大学药学院,呼和浩特010110 [4]内蒙古医科大学附属医院麻醉科,呼和浩特010110

出　　处：《中国药房》2022年第11期1348-1354,共7页China Pharmacy

基　　金：内蒙古自治区卫生健康委卫生健康科技计划项目(No.202201186);内蒙古自治区高等学校科学技术研究项目(No.NJZY22655);内蒙古自治区高等学校科学研究项目(No.NJZY21593);内蒙古医科大学实验室开放基金项目(No.2021ZN20);内蒙古医科大学科技百万工程联合项目[No.YKD2017KJBW(LH)030]。

摘　　要：目的系统研究三子散醇提物的化学组成,为阐明该方的药效物质基础提供参考。方法采用高效液相色谱-四极杆/静电场轨道阱高分辨质谱(HPLC-Q-Exactive-MS)技术进行分析。色谱条件:采用Shim-pack GIST-HP C_(18)色谱柱,以乙腈-0.1%甲酸溶液为流动相进行梯度洗脱,流速为1 mL/min,柱温为40℃,进样量为10μL。质谱条件:采用电喷雾离子源,在正、负离子检测模式下检测。检测方式为Full MS/dd-MS^(2),Full MS分辨率为70000,dd-MS^(2)分辨率为17500,扫描范围为m/z 110~1200。通过与对照品、参考文献和自建数据库信息比对后对各离子峰进行鉴定。结果在蒙药三子散醇提物中共鉴定出化合物64个,包括9个黄酮类成分、13个环烯醚萜类成分、14个有机酸类成分、18个鞣质类成分、3个三萜类成分、3个氨基酸类成分和4个脂肪酸类成分。结论本研究发现蒙药三子散醇提物中主要含环烯醚萜类、鞣质类和黄酮类成分等,可能是三子散发挥药效的物质基础。OBJECTIVE To systematically study the chemical components of ethanol extract from Sanzi san,and to provide reference for clarifying the pharmacodynamic material basis of the formulation.METHODS HPLC-Q-Exactive-MS technology was adopted.The determination was performed on Shim-pack GIST-HP C_(18) column with mobile phase consisted of acetonitrile-0.1%formic acid aqueous solution for gradient elution at the flow rate of 1 mL/min.The column temperature was 40℃,and sample size was 10μL.Mass spectrometry conditions included the electrospray spray ion source was used for detection in positive and negative ion detection modes.Full MS/dd-MS^(2)detection mode was adopted,the resolution of Full MS was 70000 and the resolution of dd-MS^(2)was 17500.The scanning range was m/z 110-1200.The ion peaks were identified by comparing with the information of control substances,literature references and self-built database.RESULTS A total of 64 components were identified in the ethanol extract of Mongolian medicine Sanzi san,including 9 flavonoids,13 iridoids,14 organic acids,18 tannins,3 triterpenes,3 amino acids and 4 fatty acids.CONCLUSIONS The ethanol extract of Mongolian medicine Sanzi san mainly include iridoids,tannins and flavonoids,which might be the pharmacodynamic material basis of Sanzi san.

关 键 词：蒙药 三子散 高效液相色谱-四极杆/静电场轨道阱高分辨质谱 成分组成 结构鉴定 

分 类 号：R931.5[医药卫生—生药学]