防治蔬菜灰霉病枯草芽胞杆菌BAB-1水分散片剂的研制  

Development of Bacillus subtilis BAB-1 Water Dispersible Tablet to Control Vegetable Gray Mold

作　　者：敖明远 张晓云[1] 郭庆港[1] 曲远航 鹿秀云[1] 崔建州[2] 李社增[1] 马平[1] AO Mingyuan;ZHANG Xiaoyun;GUO Qinggang;QU Yuanhang;LU Xiuyun;CUI Jianzhou;LI Shezeng;MA Ping(IPM Center of Hebei Province/Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China,Ministry of Agriculture and Rural Affairs/Institute of Plant Protection,Hebei Academy of Agricultural and Forestry Sciences,Baoding 071000,China;College of Forestry,Hebei Agricultural University,Baoding 071000,China)

机构地区：[1]河北省农林科学院植物保护研究所/河北省农业有害生物综合防治工程技术研究中心/农业部华北北部作物有害生物综合治理重点实验室,保定071000 [2]河北农业大学林学院,保定071000

出　　处：《中国生物防治学报》2022年第2期404-413,共10页Chinese Journal of Biological Control

基　　金：河北省重点研发计划(20326515D);河北省农林科学院现代农业科技创新工程(2019-1-2-03)。

摘　　要：枯草芽胞杆菌BAB-1是一株有效防治蔬菜灰霉病和白粉病的生防细菌。为提高该菌株的应用潜力,本研究以BAB-1菌株为有效成分开展水分散片剂的研制。通过生物相容性试验、正交试验和单因素试验对崩解剂、粘结剂及分散剂进行筛选,最终确定100亿CFU/g枯草芽胞杆菌BAB-1水分散片剂的适宜配方为酒石酸5%、碳酸氢钠25%、氯化钠0.5%、乳糖5%、PEG60002%、NNO 4%,BAB-1母药补足至100%。质量检测结果表明,该水分散片剂崩解时间为272.17 s,pH值为5.75,悬浮率为73.77%,产品各项指标均符合要求。温室盆栽试验结果表明,该制剂500倍液处理能够有效防治黄瓜与番茄灰霉病,防效分别达到84.11%和75.15%,与对照药剂43%氟菌·肟菌酯(露娜森)1000倍液的防治效果无显著性差异(76.25%与84.85%),但显著高于2亿孢子/g木霉菌可湿性粉剂500倍液(62.71%与57.95%)。Bacillus subtilis BAB-1 exhibited strong control effect on vegetable gray mold and powdery mildew.To improve the application potential,with B.subtilis BAB-1 as active ingredient,a formulation of strain BAB-1 water dispersible tablet(WT)was developed.The optimized disintegrant,binder and dispersant were screened by biocompatibility test,orthogonal test or single factor test,and the final formulation of 10 billion CFU/g B.subtilis BAB-1 WT was determined as 5%tartaric acid,25%sodium bicarbonate,0.5%sodium chloride,5%lactose,2%PEG6000,4%NNO,and up to 100%by strain BAB-1 technical material.The quality of this formulation was satisfied with dispersion time of 272.17 s,pH 5.75 and suspension rate of 73.77%.Pot test showed the 500-fold aqueous solution treatment of BAB-1 WT could effectively control gray mold on cucumber and tomato with control efficacy of 84.11%and 75.15%,respectively.There was no significant difference in the control effect between the BAB-1 WT and the 1000-fold aqueous solution of 43%trifloxystrobin fluopyram(Lunasen)(76.25%and 84.85%),but significantly higher than that of the 500-fold aqueous solution of 2×10^(8) conidia/g Trichoderma WP(62.71%and 57.95%).

关 键 词：枯草芽胞杆菌 水分散片剂 灰霉病 微生物杀菌剂 

分 类 号：S476[农业科学—农业昆虫与害虫防治]