黄瓜细菌性角斑病拮抗细菌的筛选及其防治效果  

Screening of Antagonistic Bacteria against Bacterial Angular Leaf Spot of Cucumber and Its Control Effect

作　　者：苑宝洁 李磊 张红杰[2] 石延霞[1] 柴阿丽[1] 谢学文[1] 李宝聚[1] YUAN Baojie;LI Lei;ZHANG Hongjie;SHI Yanxia;CHAI Ali;XIE Xuewen;LI Baoju(Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China;Hebei North University,Zhangjiakou 075000,China)

机构地区：[1]中国农业科学院蔬菜花卉研究所,北京100081 [2]河北北方学院,张家口075000

出　　处：《中国生物防治学报》2022年第2期421-427,共7页Chinese Journal of Biological Control

基　　金：国家重点研发计划(2017YFD0701301);中国农业科学院科技创新工程(CAAS-ASTIP-IVFCAAS);农业部园艺作物生物学与种质创制重点实验室项目;宁夏瓜菜产业协同创新中心(2017DC55)。

摘　　要：黄瓜细菌性角斑病是近年来危害严重的黄瓜病害之一,严重时可导致黄瓜大面积减产。本实验室从黄瓜根际土壤中筛选得到一株对黄瓜细菌性角斑病菌有显著抑制效果的生防细菌ZF145。经菌落形态观察、生理生化测定、Biolog测定和多基因序列分析,鉴定菌株ZF145为贝莱斯芽胞杆菌Bacillus velezensis。该菌株在平板离体试验中的抑菌圈直径为5.60 cm,抑制率为62.22%;在盆栽和田间试验中,贝莱斯芽胞杆菌ZF145对黄瓜细菌性角斑病有显著的防治效果,盆栽防效为71.10%,田间防效为61.30%。平板对峙和双层培养试验证明菌株ZF145具有较为广谱的抑菌效果,可以明显抑制6株病原细菌和8株病原真菌的生长。综上,菌株ZF145生防性状优良,在植物病害生物防治中有一定应用潜力。In recent years,bacterial angular leaf spot is one of the most serious cucumber diseases,which can lead to yield reduction in a large area.An antagonistic bacterium ZF145 with significant inhibitory effect on bacterial angular leaf spot was isolated from the rhizosphere soil of cucumber.Based on colony morphology,physiological and biochemical analysis,biolog analysis and multi-gene phylogenetic tree analysis,the strain ZF145 was identified as Bacillus velezensis.Plate inhibition test showed that the diameter of inhibition zone was 5.60 cm and the inhibition rate was 62.22%;but pot and field experiments indicated that the control effect on bacterial angular leaf spot was 71.10%and 61.30%,respectively.The antagonistic experiments showed that strain ZF145 had a broad-spectrum antibacterial effect,which can significantly inhibit the growth of six pathogenic bacteria and eight pathogenic fungi.In conclusion,strain ZF145 had excellent biocontrol properties and had a certain application potential in biological control of plant diseases.

关 键 词：黄瓜细菌性角斑病 贝莱斯芽胞杆菌ZF145 防治效果 

分 类 号：S476[农业科学—农业昆虫与害虫防治]