葡萄灰霉病生防芽胞杆菌的筛选与防效评价  

Screening of Biocontrol Bacillus spp.and Its Suppression Efficacy on Grape Gray Mold

作　　者：付莉媛 蔡瑞杰 冯志敏 宋新娜 朱英波 史凤玉 刘建斌[2] FU Liyuan;CAI Ruijie;FENG Zhimin;SONG Xinna;ZHU Yingbo;SHI Fengyu;LIU Jianbin(College of Agronomy and Biotechnology,Hebei Normal University of Science and Technology,Changli 066004,China;Institute of Plant Nutrition and Resources,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China;Hebei Key Laboratory of Crop Stress Biology(in Preparation),Hebei Normal University of Science and Technology,Qinhuangdao 066000,China)

机构地区：[1]河北科技师范学院农学与生物科技学院,昌黎066004 [2]北京市农林科学院植物营养与资源环境研究所,北京100097 [3]河北科技师范学院河北省作物逆境生物学重点实验室(筹),秦皇岛066000

出　　处：《中国生物防治学报》2022年第2期440-446,共7页Chinese Journal of Biological Control

基　　金：国家重点研发计划(2017YFD0200603);北京市农林科学院青年科研基金(QNJJ201908)。

摘　　要：本研究从新疆、山东及河北等地区采集的健康葡萄根际土壤中分离生防菌,以葡萄灰霉病菌Botrytis cinerea为靶标菌,通过平板对峙试验、葡萄叶片和果实上的离体抑菌试验,筛选获得4株生防菌BF1-1、BF2-1、BF3-1和BF4-1具有较好的拮抗作用,经形态学和分子生物学方法分别鉴定为地衣芽胞杆菌Bacillus licheniformis、解淀粉芽胞杆菌B.amyloliquefaciens、枯草芽胞杆菌B.subtilis和蜡样芽胞杆菌B.cereus;4株生防菌所制成的生物菌肥,对葡萄灰霉病的田间防效可达到66.50%~85.68%,具有开发应用潜力。Biocontrol strains were isolated from healthy grape rhizosphere soil collected from Xinjiang,Shandong,Hebei provinces.Four antagonistic strains showed good biocontrol activities against Botrytis cinerea on grape leaves and fruit by the plate test.Based on morphology and phylogenetic tree analysis,the strains(BF1-1、BF2-1、BF3-1 and BF4-1)were identified as Bacillus licheniformis,Bacillus amyloliquefaciens,Bacillus subtilis,and Bacillus cereus,respectively.The control efficacies of fertilizer made from the four strains were between 66.50%~85.68%in the field trial.

关 键 词：葡萄灰霉病 生物防治 芽胞杆菌 防效评价 

分 类 号：S476[农业科学—农业昆虫与害虫防治]