有机相生物传感器电催化反应机理及其在植物油检测中的应用  

Research on the Electro-Catalytic Reaction Mechanism of an Organic Phase Biosensor and its Application in the Vegetable Oil Detection

作　　者：陶莎[1] 于丽敏 TAO Sha;YU Limin(College of Information and Electrical Engineering,China Agricultural University,Beijing 100083;College of Information Science and Engineering,Shandong Agriculture and Engineering University,Jinan Shandong 250100)

机构地区：[1]中国农业大学信息与电气工程学院,北京100083 [2]山东农业工程学院信息科学与工程学院,山东济南250100

出　　处：《山东农业工程学院学报》2022年第5期26-35,共10页The Journal of Shandong Agriculture and Engineering University

基　　金：山东省重点研发计划项目(2019GSF111035)。

摘　　要：采用溶胶-凝胶法制备辣根过氧化物酶修饰电极,探讨了HRP酶电极在有机相中的催化反应机理和电极过程。HRP酶电极能在有机相介质中对过氧化月桂酰产生显著的还原电流响应,氰化钾作为电子介质能有效地在HRP酶与电极表面之间传递电子,增强还原电流响应。当扫描速度较低时,HRP酶电极主要涉及传质过程控制,即通过将HRP酶转移到电极表面产生还原电流来进行还原反应。当扫描速度高,电极的传质过程控制减弱,逐渐变成了表面过程控制。过氧化月桂酰在HRP酶修饰电极上的催化还原电流在0.08~6.4μg/mL浓度范围内呈线性增加(△Ipred=0.107×C+0.0119,R=0.9925)。所建立的电化学方法具有一定的重现性和准确性,但在实际植物油样品的测定中,所得结果与国标常用方法测定的结果一致性较差,总值较小。分析原因可能是植物油的加入使有机相的粘度增大,还原电流响应小于真实值。The Sol-Gel method were used to prepare the horseradish peroxidase(HRP)enzyme modified electrode,the mechanism of catalytic reaction and electrode process of the HRP enzyme electrode were explored.The HRP enzyme electrode can generate a significant reduction current response to lauroyl peroxide in an organic phase medium,and potassium ferrocyanide as the electron mediator can effectively transfer electrons between the HRP enzyme and the electrode surface,and enhance reduction current responses.When the scanning speed is low,the HRP enzyme electrode mainly involves the mass transfer process control,that is,the reduction reaction is carried out by transfer of the HRP enzyme onto the electrode surface to generate reduction current.When the scanning speed is high,the mass transfer process control of the electrode weakens and is gradually turned into the surface process control.The catalytic reduction current of lauroyl peroxide at the HRP enzyme modified electrode increases linearly with the concentration of substrate in the range of 0.08 to 6.4μg/mL(△Ipred=0.107×C+0.0119,R=0.9925).The developed electrochemical method exhibits a certain degree of reproducibility and accuracy,but in the determination of actual vegetable oil samples,the obtained results show weak consistency with the results determined by the iodometric method of the national standard,with smaller overall values.The reason may be that the addition of the vegetable oil enables the increase in the viscosity of the organic phase,and the reduction current responses are smaller than the true values.

关 键 词：有机相 电催化反应 酶修饰电极 植物油分析 

分 类 号：S132[农业科学—农业基础科学]