基于转录组的蔗茅CML基因家族鉴定及冷胁迫表达分析  

Identification and Cold Stress Expression Analysis of CML Gene Family in Erianthus fulvus Based on Transcriptome

作　　者：钱禛锋 谷书杰 赵雪婷 饶席兵 曾丹 沈庆庆 张蓉琼 陈疏影 何丽莲[1,2] 李富生[1,2] QIAN Zhen-Feng;GU Shu-Jie;ZHAO Xue-Ting;RAO Xi-Bing;ZENG Dan;SHEN Qing-Qing;ZHANG Rong-Qiong;CHEN Shu-Ying;HE Li-Lian;LI Fu-Sheng(College of Agronomy and Biotechnology,Yunnan Agricultural University,Kunming 650201,China;Sugarcane Research Institute,Yunnan Agricultural University,Kunming 650201,China)

机构地区：[1]云南农业大学农学与生物技术学院,昆明650201 [2]云南农业大学甘蔗研究所,昆明650201

出　　处：《农业生物技术学报》2022年第5期885-895,共11页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金(31960451,31560417);云南省应用基础研究计划重点项目(2015FA024)。

摘　　要：类钙调蛋白(calmodulin-like protein,CML)是植物细胞中的钙离子结合蛋白,在植物生长发育和逆境胁迫的信号转导中具有重要的功能。为探究冷胁迫下蔗茅(Erianthus fulvus)CML家族基因的表达模式,本研究基于转录组数据在蔗茅中鉴定出39个EfCML家族基因,并进行了生物信息学和冷胁迫下的表达分析。蛋白质理化性质分析表明,蔗茅EfCMLs氨基酸残基数目为59~312,分子量大小为6.24021~34.01778 kD,等电点为3.99~11.14。亚细胞定位预测结果显示,39个EfCMLs分别定位于细胞质、细胞膜和细胞核中。保守结构域分析发现,EfCML家族蛋白含有1~4个典型的EF-hand结构域。基序(motif)分析表明,EfCML家族蛋白含有1~4个典型的Ca^(2+)结合基序。进化树分析表明,EfCMLs蛋白分为11个组群。冷胁迫表达分析表明,低温诱导EfCML家族基因差异表达,其中EfCML1和EfCML2基因表达持续上调,在72 h冷胁迫下达到峰值,分别为对照的27和35倍(P<0.01),呈现最活跃状态。本研究为深入解析蔗茅CML基因的冷胁迫调控机理提供基础资料。Calmodulin-like(CML)is a calcium-binding protein in plant cells that is involved in signal transduction during growth,development,and stress.In order to explore the expression pattern of CML family genes in Erianthus fulvus under cold stress,39 EfCML family genes were identified based on transcriptome data,and bioinformatics and expression analysis under cold stress were carried out.The analysis of the physicochemical properties of the proteins showed the number of amino acid residues,molecular weight,and isoelectric point of EfCMLs proteins was 59~312,6.24021~34.01778 kD,and 3.99~11.14,respectively.The predicted results of subcellular localization showed that the 39 EfCMLs were located in the cytoplasm,cell membrane,and nucleus,respectively.Conservative domain analysis showed that EfCML family proteins contained 1~4 typical EF-hand domains.Motif analysis showed that EfCML family proteins contained 1~4 typical Ca^(2+)binding motifs.Phylogenetic tree analysis showed that EfCMLs proteins could be divided into 11 groups.Expression analysis of cold stress showed that low temperature could induce differential expression of EfCML family genes,with EfCML1 and EfCML2 were consistently up-regulated,and reached peak value at 72 h cold stress with 27 and 35 times higher than that of the control(P<0.01),showing the most active state.This study provides basic data for in-depth analysis of the cold stress regulation mechanism of E.fulvus CML genes.

关 键 词：蔗茅 类钙调蛋白(CML)家族 生物信息学分析 冷胁迫 表达分析 

分 类 号：S332.5[农业科学—作物遗传育种]