藏羊MSTN基因表达及其与产肉性状的关联性分析  

Expression of MSTN Gene and Its Association Analysis with Meat Quality in Tibetan Sheep(Ovis aries)

作　　者：张军霞 雷秀存[2] 贺娜 崔亚杰 吕彩玲 李明明 郎侠 王彩莲[1] ZHANG Jun-Xia;LEI Xiu-Cun;HE Na;CUI Ya-Jie;LYU Cai-Ling;LI Ming-Ming;LANG Xia;WANG Cai-Lian(Institute of Animal&Pasture Science and Green Agriculture/Key Laboratory for Sheep,Goat,and Cattle Germplasm and Straw Feed in Gansu Province,Gansu Academy of Agricultural Science,Lanzhou 730070,China;College of Agriculture and Animal Husbandry,Qinghai University,Xining 810016,China)

机构地区：[1]甘肃省农业科学院畜草与绿色农业研究所/甘肃省牛羊种质与秸秆饲料化重点实验室,兰州730020 [2]青海大学农牧学院,西宁810016

出　　处：《农业生物技术学报》2022年第5期926-934,共9页Journal of Agricultural Biotechnology

基　　金：甘肃省农业科学院甘肃省牛羊种质与秸秆饲料化重点实验室开放项目(2019KL01);甘肃省农业科学院科研条件建设及成果转化项目(2021GAAS01)。

摘　　要：肌肉生长抑制素(myostain,MSTN)作为肌肉生长抑制因子,属于TGF-β超家族成员,MSTN突变会引起肌肉过度增长,出现“双肌”表型,该基因对于提高动物肌肉产量及瘦肉率有重要意义。为了分析藏羊(Ovis aries)MSTN基因与产肉性状的关联性,本研究随机选取体重相近、健康无病的6只6月龄藏羊,屠宰后测定了藏羊的屠宰性能、肉品质和肉质营养成分;利用荧光定量PCR检测藏羊MSTN基因在臂三头肌、股四头肌、背最长肌和半腱肌中mRNA的表达量,并对MSTN基因表达量与产肉性状进行关联性分析。结果表明:藏羊所有屠宰性能指标在公羊与母羊之间无显著差异,公羊和母羊的屠宰率分别为51.32%和52.01%,胴体重分别为22.08和20.03 kg。肉品质分析结果表明,除了剪切力在公、母羊之间有显著差异(P<0.05)外,其余指标在公、母羊间差异均不显著。肉质营养成分分析结果中,各指标在公、母羊间差异不显著。其中公羊与母羊的蛋白质含量分别为23.14%和22.17%,粗脂肪含量分别为2.46%和2.87%。MSTN基因在藏羊4种肌肉中均有表达,在各组织间表达未达显著差异水平。对MSTN基因的表达量与屠宰性能和肉品质中各指标的相关性分析结果显示,藏羊MSTN基因在臂三头肌中的表达量与宰前活重呈显著正相关(P<0.05,1.000);在背最长肌中的表达量与红度值呈显著正相关(P<0.05,0.831),与熟肉率呈极显著负相关(P<0.01,-0.958);股四头肌中的表达量与熟肉率呈极显著负相关(P<0.01,-1.000);半腱肌中的表达量与各指标相关性均不显著。可见,MSTN基因与藏羊产肉性状存在相关性,本研究可为MSTN基因对藏羊肉品质调控机理的研究提供理论依据。As a muscle growth inhibitor,myostain(MSTN)is a member of TGF-βsuperfamily.MSTN mutation will cause excessive muscle growth and a"double muscle"phenotype.The gene is of great significance for improving muscle yield and lean meat rate of animals.The current study aimed to analyze the correlation between MSTN gene and meat production traits of Tibetan sheep(Ovis aries),six 6-month-old Tibetan sheep with similar body weight and no disease were randomly selected in this study.After slaughtering,slaughter performance,meat quality and meat nutritional components of Tibetan sheep were determined.The mRNA expression levels of MSTN gene in brachial triceps,quadriceps femoris,longissimus dorsi and semitendinosus of Tibetan sheep were detected by qPCR,and the correlation between the expression level of MSTN gene and meat production traits was analyzed.The results showed that all slaughter performance indexes were no significant difference between ram and ewe.The slaughter rates of ram and ewe were 51.32%and 52.01%,respectively,as well as the carcass weights were 22.08 and 20.03 kg,respectively.The results of meat quality analysis showed that there was no significant difference between male and female sheep except shear force(P<0.05).There were no significant differences in the contents of crude protein,crude fat,crude ash and water between ram and ewe.The protein contents were 23.14%and 22.17%for ram and ewe,respectively,and the crude fat contents of ram and ewe were 2.46%and 2.87%,respectively.MSTN gene was expressed in all muscle tissues of Tibetan sheep,but the differences of the expression levels in all muscle tissues were not significant.By analyzing the correlation between the expression level of MSTN gene and each index,the mRNA expression level of MSTN gene in arm triceps of Tibetan sheep was significantly positively correlated with the live weight before slaughter(P<0.05,1.000);The expression level in longissimus dorsi muscle was significantly positively correlated with the redness value(P<0.05,0.831),and it was e

关 键 词：肌肉生长抑制素基因(MSTN) 藏羊 屠宰性能 肉品质 基因表达 

分 类 号：S811[农业科学—畜牧学]