生晒参蛋白质对乳腺癌MCF-7细胞的抑制作用及其参与凋亡和迁移途径的调控机制  

Inhibition of Suncured Panax ginseng Protein on Breast Cancer MCF-7 Cells and Its Regulation Mechanism in Apoptosis and Migration Pathway

作　　者：万茜淋 任雨贺 吴新民[4] 蔺冬梅 杨洪梅 刘馨泽 冯琳 刘淑莹 王琦[2] 李玉[2] 陈长宝 WAN Xi-lin;REN Yu-he;WU Xin-min;LIN Dong-mei;YANG Hong-mei;LIU Xin-ze;FENG Lin;LIU Shu-ying;WANG Qi;LI Yu;CHEN Chang-bao(Jilin Province Institution of Ginseng Science,Changchun University of Chinese Medicine,Changchun 130117,China;Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi,Jilin Agricultural Univer-sity,Changchun 130118,China;Institute of Special Animal and Plant Sciences of Chinese Academy of Sciences,Chan-gchun 130112,China;Department of Neurosurgery,the First Hospital of Jilin University,Changchun 130021,China;Electric diagnosis Department,Jilin Municipal Hospital of Traditional Chinese Medicine,Jilin 132000,China)

机构地区：[1]长春中医药大学吉林省人参科学研究院,吉林长春130117 [2]吉林农业大学食药用菌教育部工程研究中心,吉林长春130118 [3]中国农业科学院特产研究所,吉林长春130112 [4]吉林大学第一医院神经肿瘤外科,吉林长春130021 [5]吉林市中医院电诊科,吉林吉林132000

出　　处：《特产研究》2022年第3期1-5,12,共6页Special Wild Economic Animal and Plant Research

基　　金：吉林省教育厅科学技术研究项目(JJKH20220876KJ);吉林省中医药管理局科技项目(2021003);国家自然科学基金面上项目(82073969);吉林省科技重大专项(20200504003 YY)。

摘　　要：本研究以人乳腺癌MCF-7细胞为模型,探究生晒参蛋白质的抗肿瘤活性及作用机制。根据前期研究结果,采用半数抑制浓度(IC50)对乳腺癌细胞MDA-MB-231、MCF-7和人正常乳腺上皮细胞MCF10A进行干预,采用CCK-8法测定细胞活性,并计算细胞抑制率;利用细胞划痕实验检测SGP在不同浓度和时间点对MCF-7细胞迁移能力的影响;通过Transwell小室实验检测生晒参蛋白对MCF-7细胞侵袭能力的干预作用;并采用Q-PCR和Western Blotting方法检测生晒参蛋白作用48 h后,MCF-7细胞的凋亡基因以及凋亡和迁移相关蛋白的表达情况。结果显示,生晒参蛋白质能显著抑制乳腺癌细胞的增殖,分别作用24 h、48 h和72h后细胞迁移能力受到显著抑制;生晒参蛋白质通过调控凋亡蛋白Bax和Bcl-2的基因和蛋白的表达,诱导MCF-7细胞的凋亡,并且通过抑制MCF-7细胞基质金属蛋白酶家族MMP-2和MMP-9的表达,参与调控肿瘤细胞的迁移和侵袭。生晒参蛋白质通过调控凋亡相关基因和蛋白的表达以及基质金属蛋白酶家族成员的表达水平,可有效抑制人乳腺癌MCF-7细胞的迁移,并促进细胞的凋亡。Human breast cancer MCF-7 cells were used as a model to explore the antitumor activity and mechanism of the protein of suncured Panax ginseng. According to the results of previous studies, half inhibitory concentration(IC50) was used to intervene breast cancer cell MDA-MB-231, MCF-7 and breast tissue MCF10A, cell activity was measured by CCK-8 method, and cell inhibition rate was calculated.Effects of cell scratch test on cell migration of MCF-7 cells after 24 h, 48 h and 72 h of drug administration;Transwell chamber assay was used to detect the intervention effect of SGP on the invasion ability of MCF-7 cells. Q-PCR and Western Blotting were used to detect the expression of apoptotic genes and apoptotic and migration-related proteins in MCF-7 cells after 48h treatment. The results showed that suncured Panax ginseng protein could significantly inhibit the proliferation of breast cancer MCF-7 cells, and the cell migration ability was significantly inhibited after treatment for 24, 48 and 72h. Apoptosis of MCF-7 cells was induced by the regulation of the expression of Bax and Bcl-2 genes. In addition, MCF-7 cells participate in the regulation of tumor cell migration and invasion by inhibiting the expression of MMP-2 and MMP-9, the matrix metalloproteinase family. It was concluded that the expression of apoptosis-related genes and proteins, as well as the expression of matrix metalloproteinase family members, can effectively inhibit the migration of human breast cancer MCF-7 cells and promote cell apoptosis.

关 键 词：人参蛋白质 人乳腺癌MCF-7细胞 细胞迁移 作用机制 

分 类 号：S567.51[农业科学—中草药栽培] R285[农业科学—作物学]